TY - JOUR
T1 - NMDA receptor-mediated immediate Ser831 phosphorylation of GluR1 through CaMKIIα in rat hippocampus during early global ischemia
AU - Fu, Xin Zhen
AU - Zhang, Quan Guang
AU - Meng, Fan Jie
AU - Zhang, Guang Yi
N1 - Funding Information:
This work was supported by National Natural Science Foundation of China (nos. 30170220 and 30070182). The authors would like to thank Dr. Yong Liu for his assistance.
PY - 2004/1
Y1 - 2004/1
N2 - The phosphorylation of α-amino-3-hydroxy-5-methylisoxazole-4- propionic acid (AMPA) receptors subunit GluR1 at Ser831 has been implicated in the regulation of AMPA receptors channel. In this paper, Ser831 phosphorylation of GluR1 in rat hippocampus was investigated, which significantly increased during early global ischemia. To further illustrate the underlying mechanisms, calcium/calmodulin-dependent kinase IIα (CaMKIIα) inhibitor 1-[N,O-bis-(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine (KN62), CaM antagonist trifluoperazine (TFP), N-methyl-D-aspartate (NMDA) receptor antagonist dextromethorphan (DEX), AMPA receptor antagonist 6,7-dinitro-quinoxaline-2,3-(1H,4H)-dione (DNQX) and L-type voltage-gated Ca2+ channel (L-VGCC) blocker nifedipine (NIF), were respectively administrated to the rats 20min prior to ischemia. The results showed that KN62, TFP and DEX significantly attenuated Ser831 phosphorylation of GluR1, while DNQX and NIF had no obvious effects. Consequently, the studies suggest that early global ischemia induced Ser831 phosphorylation of GluR1 may be closely associated with CaMKIIα and the NMDA receptor, while the immediate Ser831 phosphorylation of GluR1 may have been involved in pathogenic events after early global ischemia.
AB - The phosphorylation of α-amino-3-hydroxy-5-methylisoxazole-4- propionic acid (AMPA) receptors subunit GluR1 at Ser831 has been implicated in the regulation of AMPA receptors channel. In this paper, Ser831 phosphorylation of GluR1 in rat hippocampus was investigated, which significantly increased during early global ischemia. To further illustrate the underlying mechanisms, calcium/calmodulin-dependent kinase IIα (CaMKIIα) inhibitor 1-[N,O-bis-(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine (KN62), CaM antagonist trifluoperazine (TFP), N-methyl-D-aspartate (NMDA) receptor antagonist dextromethorphan (DEX), AMPA receptor antagonist 6,7-dinitro-quinoxaline-2,3-(1H,4H)-dione (DNQX) and L-type voltage-gated Ca2+ channel (L-VGCC) blocker nifedipine (NIF), were respectively administrated to the rats 20min prior to ischemia. The results showed that KN62, TFP and DEX significantly attenuated Ser831 phosphorylation of GluR1, while DNQX and NIF had no obvious effects. Consequently, the studies suggest that early global ischemia induced Ser831 phosphorylation of GluR1 may be closely associated with CaMKIIα and the NMDA receptor, while the immediate Ser831 phosphorylation of GluR1 may have been involved in pathogenic events after early global ischemia.
KW - Calcium/calmodulin-dependent kinase IIα
KW - GluR1
KW - Ischemia
KW - N-Methyl-D-aspartate receptor
KW - Phosphorylation
KW - α-Amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor
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U2 - 10.1016/j.neures.2003.09.009
DO - 10.1016/j.neures.2003.09.009
M3 - Article
C2 - 14687884
AN - SCOPUS:0347626205
SN - 0168-0102
VL - 48
SP - 85
EP - 91
JO - Neuroscience Research
JF - Neuroscience Research
IS - 1
ER -