TY - JOUR
T1 - Novel multifunctional nanocomposite for root caries restorations to inhibit periodontitis-related pathogens
AU - Xiao, Shimeng
AU - Wang, Haohao
AU - Liang, Kunneng
AU - Tay, Franklin Chi Meng
AU - Weir, Michael D.
AU - Melo, Mary Anne S.
AU - Wang, Lin
AU - Wu, Yafei
AU - Oates, Thomas W.
AU - Ding, Yi
AU - Xu, Hockin H.K.
N1 - Funding Information:
This work was supported by National Natural Science Foundation of China81141062 (Y.D.) and 81800965 (L.K.N), Fundamental Research Funds for Central University2018SCU12016 (L.K.N), Research Fund of West China HospitalWCHS-201705 (L.K.N), University of Maryland School of Dentistry bridging fund (H.H.K.X.), and University of Maryland seed grant (H.H.K.X.)
Funding Information:
This work was supported by National Natural Science Foundation of China 81141062 (Y.D.) and 81800965 (L.K.N), Fundamental Research Funds for Central University 2018SCU12016 (L.K.N), Research Fund of West China Hospital WCHS-201705 (L.K.N), University of Maryland School of Dentistry bridging fund (H.H.K.X.), and University of Maryland seed grant (H.H.K.X.)
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2019/2
Y1 - 2019/2
N2 - Objectives: The objectives of this study were to: (1) develop a novel multifunctional composite with nanoparticles of silver (NAg), 2-methacryloyloxyethyl phosphorylcholine (MPC), dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP); and (2) investigate biofilm-inhibition via the multifunctional nanocomposite against three species of periodontal pathogens for the first time. Methods: The multifunctional nanocomposite was fabricated by incorporating NAg, MPC, DMAHDM and NACP into the resin consisting of pyromellitic glycerol dimethacrylate (PMDGDM) and ethoxylated bisphenol A dimethacrylate (EBPADMA). Three species (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum) were tested for metabolic activity (MTT), live/dead staining, polysaccharide production and colony-forming units (CFU) of biofilms grown on resins. Results: Incorporation of 0.08% to 0.12% NAg, 3% MPC, 3% DMAHDM and 30% NACP did not compromise the mechanical properties of the composite (p > 0.1). The multifunctional nanocomposite reduced protein adsorption to nearly 1/10 of that of a commercial control (p < 0.05). For all three species, the biofilm CFU was reduced by about 5 and 1 orders of magnitude via the nanocomposite containing NAg + MPC + DMAHDM, compared to commercial control and the composite with MPC + DMAHDM, respectively. Conclusions: The novel multifunctional nanocomposite achieved the greatest reduction in metabolic activity, polysaccharide and biofilm growth of three periodontal pathogens. Clinical significance: The strongly-antibacterial, multifunctional composite is promising for treating root lesions, alleviating periodontitis and protecting the periodontal tissues.
AB - Objectives: The objectives of this study were to: (1) develop a novel multifunctional composite with nanoparticles of silver (NAg), 2-methacryloyloxyethyl phosphorylcholine (MPC), dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP); and (2) investigate biofilm-inhibition via the multifunctional nanocomposite against three species of periodontal pathogens for the first time. Methods: The multifunctional nanocomposite was fabricated by incorporating NAg, MPC, DMAHDM and NACP into the resin consisting of pyromellitic glycerol dimethacrylate (PMDGDM) and ethoxylated bisphenol A dimethacrylate (EBPADMA). Three species (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum) were tested for metabolic activity (MTT), live/dead staining, polysaccharide production and colony-forming units (CFU) of biofilms grown on resins. Results: Incorporation of 0.08% to 0.12% NAg, 3% MPC, 3% DMAHDM and 30% NACP did not compromise the mechanical properties of the composite (p > 0.1). The multifunctional nanocomposite reduced protein adsorption to nearly 1/10 of that of a commercial control (p < 0.05). For all three species, the biofilm CFU was reduced by about 5 and 1 orders of magnitude via the nanocomposite containing NAg + MPC + DMAHDM, compared to commercial control and the composite with MPC + DMAHDM, respectively. Conclusions: The novel multifunctional nanocomposite achieved the greatest reduction in metabolic activity, polysaccharide and biofilm growth of three periodontal pathogens. Clinical significance: The strongly-antibacterial, multifunctional composite is promising for treating root lesions, alleviating periodontitis and protecting the periodontal tissues.
KW - Antibacterial activity
KW - Class V restorations
KW - Multifunctional nanocomposite
KW - Periodontal pathogens
KW - Root caries
KW - Silver nanoparticle
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U2 - 10.1016/j.jdent.2018.12.001
DO - 10.1016/j.jdent.2018.12.001
M3 - Article
C2 - 30552930
AN - SCOPUS:85059842862
SN - 0300-5712
VL - 81
SP - 17
EP - 26
JO - Journal of Dentistry
JF - Journal of Dentistry
ER -