TY - JOUR
T1 - NSAIDs acutely inhibit TRPC channels in freshly isolated rat glomeruli
AU - Ilatovskaya, Daria V.
AU - Levchenko, Vladislav
AU - Ryan, Robert P.
AU - Cowley, Allen W.
AU - Staruschenko, Alexander
N1 - Funding Information:
This research was supported by American Diabetes Association Grant 1-10-BS-168 , and American Physiological Society S&R Foundation Ryuji Ueno and Lazaro J. Mandel Awards (to A. Staruschenko) and the NIH Grants HL-081091 and HL-29587 (to A.W. Cowley, Jr.).
PY - 2011/5/6
Y1 - 2011/5/6
N2 - Using a novel approach for analysis of TRPC channel activity, we report here that NSAIDs are involved into regulation of TRPC channels in the podocytes of the freshly isolated decapsulated glomeruli. Fluorescence and electron microscopy techniques confirmed the integrity of podocytes in the glomeruli. Western blotting showed that TRPC1, 3 and 6 are highly expressed in the glomeruli. Single-channel patch clamp analysis revealed cation currents with distinct TRPC properties. This is the first report describing single TRPC-like currents in glomerular podocytes. Furthermore, our data provide a novel mechanism of NSAIDs regulation of TRPC channels, which might be implicated in maintaining the glomerular filtration barrier.
AB - Using a novel approach for analysis of TRPC channel activity, we report here that NSAIDs are involved into regulation of TRPC channels in the podocytes of the freshly isolated decapsulated glomeruli. Fluorescence and electron microscopy techniques confirmed the integrity of podocytes in the glomeruli. Western blotting showed that TRPC1, 3 and 6 are highly expressed in the glomeruli. Single-channel patch clamp analysis revealed cation currents with distinct TRPC properties. This is the first report describing single TRPC-like currents in glomerular podocytes. Furthermore, our data provide a novel mechanism of NSAIDs regulation of TRPC channels, which might be implicated in maintaining the glomerular filtration barrier.
KW - FSGS
KW - Focal segmental glomerulosclerosis
KW - Podocyte
KW - TRPC6
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U2 - 10.1016/j.bbrc.2011.04.005
DO - 10.1016/j.bbrc.2011.04.005
M3 - Article
C2 - 21473850
AN - SCOPUS:79955662243
SN - 0006-291X
VL - 408
SP - 242
EP - 247
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -