Abstract
Objective: To investigate the arrangement of chromosomes within pronuclei-stage mouse zygotes. Design: In vitro study. Setting: Academic medical center.Patient(s): None.Intervention(s): None. Main Outcome Measure(s): Location of major α-satellite DNA, centromeres, and telomeres, and relative location of chromosomes.Result(s): Chromosomes appeared to be oriented inward by centromeres and to be interconnected by major α-satellite DNA, which appeared to be the sole DNA component of the nucleoli. This chromosomal arrangement persisted throughout interphase. Chromosomal painting failed to identify chromosomal ordering within pronuclei.Conclusion(s): Pronuclear nucleoli are represented by α-satellite sequences of interconnecting chromosomes that hold all chromosomes together during interphase. Chromosomes within the pronucleus are randomly positioned relative to each other. Copyright (C) 2000 American Society for Reproductive Medicine.
Original language | English (US) |
---|---|
Pages (from-to) | 366-371 |
Number of pages | 6 |
Journal | Fertility and sterility |
Volume | 73 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2000 |
Externally published | Yes |
Keywords
- Centromeres
- Nucleoli
- Telomeres
- Zygote
- α-satellite
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology