Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: Close homology to placental alkaline phosphatase

P. S. Henthorn; M. Raducha; Y. H. Edwards; M. J. Weiss; C. Slaughter; M. A. Lafferty; H. Harris

doi:10.1073/pnas.84.5.1234

Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: Close homology to placental alkaline phosphatase

P. S. Henthorn, M. Raducha, Y. H. Edwards, M. J. Weiss, C. Slaughter, M. A. Lafferty, H. Harris

Research output: Contribution to journal › Article › peer-review

128 Scopus citations

Abstract

A cDNA probe for human adult intestinal alkaline phosphatase (ALP) [orthophosphoric-monoester phosphohydrolase (alkaline optimum); EC 3.1.3.1] was isolated from a λgt11 expression library. The cDNA insert of this close is 2513 base pairs in length and contains an open reading frame that encodes a 528-amino acid polypeptide. This deduced polypeptide contains the first 40 amino acids of human intestinal ALP, as determined by direct protein sequencing. Intestinal ALP shows 86.5% amino acid identity to placental (type 1) ALP and 56.6% amino acid identity to liver/bone/kidney ALP. In the 3'-untranslated regions, intestinal and placental ALP cDNAs are 73.5% identical (excluding gaps). The evolution of this multigene enzyme family is discussed.

Original language	English (US)
Pages (from-to)	1234-1238
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	84
Issue number	5
DOIs	https://doi.org/10.1073/pnas.84.5.1234
State	Published - 1987
Externally published	Yes

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Henthorn, P. S., Raducha, M., Edwards, Y. H., Weiss, M. J., Slaughter, C., Lafferty, M. A., & Harris, H. (1987). Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: Close homology to placental alkaline phosphatase. Proceedings of the National Academy of Sciences of the United States of America, 84(5), 1234-1238. https://doi.org/10.1073/pnas.84.5.1234

Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: Close homology to placental alkaline phosphatase. / Henthorn, P. S.; Raducha, M.; Edwards, Y. H. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 5, 1987, p. 1234-1238.

Research output: Contribution to journal › Article › peer-review

Henthorn, PS, Raducha, M, Edwards, YH, Weiss, MJ, Slaughter, C, Lafferty, MA & Harris, H 1987, 'Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: Close homology to placental alkaline phosphatase', Proceedings of the National Academy of Sciences of the United States of America, vol. 84, no. 5, pp. 1234-1238. https://doi.org/10.1073/pnas.84.5.1234

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AU - Slaughter, C.

AU - Lafferty, M. A.

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Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: Close homology to placental alkaline phosphatase

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