Abstract
Insulin-like growth factor binding protein-3 (IGFBP-3) gene is a structural gene associated with the growth and development of the animals. The present investigation was carried out to unravel nucleotide sequence and polymerase chain reaction-restriction fragment polymorphism (PCR-RPLP) of IGFBP-3 gene in buffalo. Genomic DNA was isolated from a total of 157 animals belonging to Murrah, Surti, Jaffarabadi and Nagpuri breeds of Indian riverine buffalo. A 655 bp of IGFBP-3 gene was amplified in all the breeds and amplicons were digested with Hae III, Taq I and Msp I restriction enzymes. On digestion with Hae III yielded single restriction pattern of 8 fragments of sizes 201, 165, 154, 56, 36, 19, 16 and 8 bp in all the animals studied. Similarly Taq I and Msp I also revealed single restriction pattern yielding fragments of sizes 240 and 415 bp and 145 and 510 bp, respectively. This shows non-polymorphic nature of restriction sites in buffalo. Nucleotide sequencing of 587 bp of IGFBP-3 gene in Murrah buffalo was done and submitted to the GenBank (Accession No. AY304829). Nucleotide sequencing revealed an addition of 4 bases in the intronic region as compared to cattle.
Original language | English (US) |
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Pages (from-to) | 910-913 |
Number of pages | 4 |
Journal | Asian-Australasian Journal of Animal Sciences |
Volume | 17 |
Issue number | 7 |
DOIs | |
State | Published - Jul 2004 |
Keywords
- Buffalo
- IGFBP-3
- PCR-RPLP
- Polymorphism
- Sequencing
ASJC Scopus subject areas
- Food Science
- Animal Science and Zoology