TY - JOUR
T1 - Opsin synthesis in the c57BL/6-mivit/mivit mouse model of retinal degeneration
AU - Smith, Sylvia B.
AU - McCoy, Judy R.
AU - Cope, Bruce K.
N1 - Funding Information:
This research was supported by NEI grant EY09682.
Funding Information:
ACKNOWLEDGMENTS The C57BL/6-mi'"/mi''' mice used in this study were the offspring from our colony of breeding pairs which were provided by Dr. R. L. Sidman, New Eng. Regional Primate Res. Ctr., Southboro, MA (funded by NEI grant EY 06859). The authors thank Mr. Ellis Cobb for care of the animals and Ms. M. J. Smith for assistance with data collection.
PY - 1993
Y1 - 1993
N2 - The capacity of photoreceptor cells to synthesize opsin was evaluated in a newly-described mouse model of retinal degeneration, the C57BL/6-mivtt/mivit. The mivit/mivit mouse loses photoreceptor cells at a rate of about one row per month beginning at 8 weeks, ROS are severely disrupted at 4 months, RPE is unevenly pigmented. Retinas of affected and control mice ages 4, 6, 8, 12, 16, 20, 24, 28, 32 and 52 weeks were incubated for 2 hours in medium containing [3H] leucine. Homogenates of retina samples were subjected to SDS-PAGE using disc gels. The gels were sliced and counted by scintillation. The incorporation of [3H] leucine into opsin was compared with its incorporation into other retinal proteins. During the early time points studied, mivit/mivit retinas incorporated proportionately similar amounts of [3H] leucine into opsin versus other retinal proteins as did controls. At 12 weeks, the percentage was about 80% and it continued to decline over the succeeding weeks studied. By 1 year, the proportion of leucine incorporated into opsin versus other proteins was only about 23 % the amount incorporated in controls. The results of the present study suggest that the mivit/mivit photoreceptor cells are able to synthesize opsin and the gradual decline in synthetic ability follows the gradual loss of cells and is not correlated with the disruption of ROS.
AB - The capacity of photoreceptor cells to synthesize opsin was evaluated in a newly-described mouse model of retinal degeneration, the C57BL/6-mivtt/mivit. The mivit/mivit mouse loses photoreceptor cells at a rate of about one row per month beginning at 8 weeks, ROS are severely disrupted at 4 months, RPE is unevenly pigmented. Retinas of affected and control mice ages 4, 6, 8, 12, 16, 20, 24, 28, 32 and 52 weeks were incubated for 2 hours in medium containing [3H] leucine. Homogenates of retina samples were subjected to SDS-PAGE using disc gels. The gels were sliced and counted by scintillation. The incorporation of [3H] leucine into opsin was compared with its incorporation into other retinal proteins. During the early time points studied, mivit/mivit retinas incorporated proportionately similar amounts of [3H] leucine into opsin versus other retinal proteins as did controls. At 12 weeks, the percentage was about 80% and it continued to decline over the succeeding weeks studied. By 1 year, the proportion of leucine incorporated into opsin versus other proteins was only about 23 % the amount incorporated in controls. The results of the present study suggest that the mivit/mivit photoreceptor cells are able to synthesize opsin and the gradual decline in synthetic ability follows the gradual loss of cells and is not correlated with the disruption of ROS.
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U2 - 10.3109/02713689308995766
DO - 10.3109/02713689308995766
M3 - Article
C2 - 8222731
AN - SCOPUS:0027304931
SN - 0271-3683
VL - 12
SP - 711
EP - 717
JO - Current Eye Research
JF - Current Eye Research
IS - 8
ER -