Optical tissue clearing in combination with perfusion and immunofluorescence for placental vascular imaging

Maira Carrillo; Marcel Chuecos; Kushal Gandhi; Andrey Bednov; David Lee Moore; James Maher; Gary Ventolini; Guangchen Ji; Natalia Schlabritz-Loutsevitch

doi:10.1097/MD.0000000000012392

Optical tissue clearing in combination with perfusion and immunofluorescence for placental vascular imaging

Maira Carrillo, Marcel Chuecos, Kushal Gandhi, Andrey Bednov, David Lee Moore, James Maher, Gary Ventolini, Guangchen Ji, Natalia Schlabritz-Loutsevitch

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Imaging of placental tissues is a difficult task, because of specific for this organ complex multicellular and 3D tissue structure. The tissue clearing systems (X-CLARITY) system is a valuable tool for the examining the expression of molecular pathways in whole tissues and organs, originally developed for brain imaging. In the present report, we utilized this technology for the examination of placental vasculature and protein expression in perfused human placental tissue. The placental tissue was sufficiently cleared with preservation of endothelial staining and fluorescent markers, allowing visualization using confocal microscopy. The CLARITY method and X-CLARITY system is a valuable tool in placental imaging.

Original language	English (US)
Article number	e12392
Journal	Medicine (United States)
Volume	97
Issue number	39
DOIs	https://doi.org/10.1097/MD.0000000000012392
State	Published - 2018
Externally published	Yes

Keywords

3D
Image clearing
Perfusion
Placenta
Vasculature

ASJC Scopus subject areas

General Medicine

Access to Document

10.1097/MD.0000000000012392

Cite this

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title = "Optical tissue clearing in combination with perfusion and immunofluorescence for placental vascular imaging",

abstract = "Imaging of placental tissues is a difficult task, because of specific for this organ complex multicellular and 3D tissue structure. The tissue clearing systems (X-CLARITY) system is a valuable tool for the examining the expression of molecular pathways in whole tissues and organs, originally developed for brain imaging. In the present report, we utilized this technology for the examination of placental vasculature and protein expression in perfused human placental tissue. The placental tissue was sufficiently cleared with preservation of endothelial staining and fluorescent markers, allowing visualization using confocal microscopy. The CLARITY method and X-CLARITY system is a valuable tool in placental imaging.",

keywords = "3D, Image clearing, Perfusion, Placenta, Vasculature",

author = "Maira Carrillo and Marcel Chuecos and Kushal Gandhi and Andrey Bednov and Moore, {David Lee} and James Maher and Gary Ventolini and Guangchen Ji and Natalia Schlabritz-Loutsevitch",

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T1 - Optical tissue clearing in combination with perfusion and immunofluorescence for placental vascular imaging

AU - Carrillo, Maira

AU - Chuecos, Marcel

AU - Gandhi, Kushal

AU - Bednov, Andrey

AU - Moore, David Lee

AU - Maher, James

AU - Ventolini, Gary

AU - Ji, Guangchen

AU - Schlabritz-Loutsevitch, Natalia

N1 - Publisher Copyright: Copyright © 2018 the Author(s). Published by Wolters Kluwer Health, Inc. This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0

PY - 2018

Y1 - 2018

N2 - Imaging of placental tissues is a difficult task, because of specific for this organ complex multicellular and 3D tissue structure. The tissue clearing systems (X-CLARITY) system is a valuable tool for the examining the expression of molecular pathways in whole tissues and organs, originally developed for brain imaging. In the present report, we utilized this technology for the examination of placental vasculature and protein expression in perfused human placental tissue. The placental tissue was sufficiently cleared with preservation of endothelial staining and fluorescent markers, allowing visualization using confocal microscopy. The CLARITY method and X-CLARITY system is a valuable tool in placental imaging.

AB - Imaging of placental tissues is a difficult task, because of specific for this organ complex multicellular and 3D tissue structure. The tissue clearing systems (X-CLARITY) system is a valuable tool for the examining the expression of molecular pathways in whole tissues and organs, originally developed for brain imaging. In the present report, we utilized this technology for the examination of placental vasculature and protein expression in perfused human placental tissue. The placental tissue was sufficiently cleared with preservation of endothelial staining and fluorescent markers, allowing visualization using confocal microscopy. The CLARITY method and X-CLARITY system is a valuable tool in placental imaging.

KW - 3D

KW - Image clearing

KW - Perfusion

KW - Placenta

KW - Vasculature

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Optical tissue clearing in combination with perfusion and immunofluorescence for placental vascular imaging

Abstract

Keywords

ASJC Scopus subject areas

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