Optimizing dentin bond durability: Control of collagen degradation by matrix metalloproteinases and cysteine cathepsins

Leo Tjäderhane, Fabio D. Nascimento, Lorenzo Breschi, Annalisa Mazzoni, Ivarne L.S. Tersariol, Saulo Geraldeli, Arzu Tezvergil-Mutluay, Marcela R. Carrilho, Ricardo M. Carvalho, Franklin R. Tay, David H. Pashley

Research output: Contribution to journalArticlepeer-review

348 Scopus citations


Objectives: Contemporary adhesives lose their bond strength to dentin regardless of the bonding system used. This loss relates to the hydrolysis of collagen matrix of the hybrid layers. The preservation of the collagen matrix integrity is a key issue in the attempts to improve the dentin bonding durability. Methods: Dentin contains collagenolytic enzymes, matrix metalloproteinases (MMPs) and cysteine cathepsins, which are responsible for the hydrolytic degradation of collagen matrix in the bonded interface. Results: The identities, roles and function of collagenolytic enzymes in mineralized dentin has been gathered only within last 15 years, but they have already been demonstrated to have an important role in dental hard tissue pathologies, including the degradation of the hybrid layer. Identifying responsible enzymes facilitates the development of new, more efficient methods to improve the stability of dentin-adhesive bond and durability of bond strength. Significance: Understanding the nature and role of proteolytic degradation of dentin-adhesive interfaces has improved immensely and has practically grown to a scientific field of its own within only 10 years, holding excellent promise that stable resin-dentin bonds will be routinely available in a daily clinical setting already in a near future.

Original languageEnglish (US)
Pages (from-to)116-135
Number of pages20
JournalDental Materials
Issue number1
StatePublished - Jan 2013


  • Adhesive
  • Chlorhexidine
  • Collagen
  • Composite resin
  • Cysteine cathepsin
  • Degradation
  • Dentin
  • Durability
  • Matrix metalloproteinase
  • Tooth

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials


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