TY - JOUR
T1 - Overexpression of anti-apoptotic gene BAG-1 in human cervical cancer
AU - Yang, Xiaolong
AU - Hao, Yawei
AU - Ferenczy, Alex
AU - Tang, Shou-Ching
AU - Pater, Alan
N1 - Funding Information:
We thank G. Chernenko for excellent technical assistance. The investigation was supported by grants from the Medical Research Council of Canada, a grant from the National Cancer Institute of Canada with funds from the Canadian Cancer Society, and a grant from the Newfoundland Cancer Treatment and Research Foundation.
PY - 1999/2/25
Y1 - 1999/2/25
N2 - Apoptosis is a programmed cell death process in which cells commit suicide under certain environmental conditions. Recent studies suggest that apoptosis is controlled by a variety of cellular genes, and dysregulation of these genes plays an important role in the pathogenesis of human diseases, including cancer. BAG-1 is a novel anti-apoptotic protein isolated by its interaction with another anti-apoptotic protein, Bcl-2. It binds to several hormone receptors and growth factor receptors and modulates their function in apoptosis. However, the role of BAG-1 in the oncogenesis of human cervical cancer has yet to be illustrated. In this study, we examined the expression of BAG-1 in cervical normal and carcinoma cultured cells and tissues. BAG-1 was overexpressed in human cervical carcinoma cell lines and tissues. Overexpression was regulated at the transcriptional level. The increased expression of BAG-1 was correlated with enhanced resistance of cervical carcinoma cells to apoptosis induced by a DNA-damaging reagent. In addition, overexpression of BAG-1 enhanced the resistance of cervical cells to apoptosis. This study provided the first evidence that BAG-1 is upregulated in human cervical cancer and may play an important role in apoptosis and human cervical carcinogenesis.
AB - Apoptosis is a programmed cell death process in which cells commit suicide under certain environmental conditions. Recent studies suggest that apoptosis is controlled by a variety of cellular genes, and dysregulation of these genes plays an important role in the pathogenesis of human diseases, including cancer. BAG-1 is a novel anti-apoptotic protein isolated by its interaction with another anti-apoptotic protein, Bcl-2. It binds to several hormone receptors and growth factor receptors and modulates their function in apoptosis. However, the role of BAG-1 in the oncogenesis of human cervical cancer has yet to be illustrated. In this study, we examined the expression of BAG-1 in cervical normal and carcinoma cultured cells and tissues. BAG-1 was overexpressed in human cervical carcinoma cell lines and tissues. Overexpression was regulated at the transcriptional level. The increased expression of BAG-1 was correlated with enhanced resistance of cervical carcinoma cells to apoptosis induced by a DNA-damaging reagent. In addition, overexpression of BAG-1 enhanced the resistance of cervical cells to apoptosis. This study provided the first evidence that BAG-1 is upregulated in human cervical cancer and may play an important role in apoptosis and human cervical carcinogenesis.
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U2 - 10.1006/excr.1998.4349
DO - 10.1006/excr.1998.4349
M3 - Article
C2 - 10047462
AN - SCOPUS:0033602024
SN - 0014-4827
VL - 247
SP - 200
EP - 207
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -