Oxygen derived radicals related injury in the heart during calcium paradox

Muhammad Ashraf

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


The effects of oxygen-derived radical scavengers (ODRS) on the heart was investigated during the calcium paradox. Perfusion with Ca2 +-free medium caused cell separation at the intercalated discs and changes in the endothelial cells. Upon Ca2+ reintroduction, a massive cell damage occurred. The cytosolic enzyme, creatine phosphokinase (CPK), was released in large amounts (p< 0.001). The tissue adenosine triphosphate (ATP) was reduced to 3.7 μmol/g dry weight from the control value of 21.6 μmol/g dry weight and tissue Ca2+ content was increased threefold. The treatment with Superoxide dismutase (SOD) and catalase (CAT) increased percentage of normal cells (62.2%) compared to nontreated Ca2+ paradox group (0.2%) and caused negligible leakage of CPK. Tissue ATP was preserved (p<0.03), and Ca2+ content was also reduced in the hearts treated with SOD and CAT (p<0.03). The cell membranes and vascular endothelium were well preserved in the hearts treated with SOD and CAT. Boiled SOD and CAT administered were totally ineffective. It is suggested that oxygen-active species may have a role in the Ca2+ paradox injury.

Original languageEnglish (US)
Pages (from-to)27-37
Number of pages11
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Issue number1
StatePublished - Dec 1987
Externally publishedYes


  • ATP
  • Ca
  • Calcium paradox
  • Catalase
  • Cell membrane
  • Cytochrome C
  • Heart
  • Oxygen derived radicals
  • Superoxide anion
  • Superoxide dismutase
  • Vascular endothelium

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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