TY - JOUR
T1 - Periodontal repair in dogs
T2 - rhBMP-2 significantly enhances bone formation under provisions for guided tissue regeneration
AU - Wikesjö, Ulf M E
AU - Xiropaidis, Andreas V.
AU - Thomson, Robert C.
AU - Cook, Alonzo D.
AU - Selvig, Knut A.
AU - Hardwick, W. Ross
PY - 2003/8
Y1 - 2003/8
N2 - Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) has been shown to support the regeneration of alveolar bone and periodontal attachment in surgically created periodontal defects and in defects with a history of dental plaque and calculus exposure. Periodontal regeneration has also been shown following guided tissue regeneration using space-providing expanded polytetrafluoroethylene (ePTFE) devices. The objective of this study was to evaluate the influence of rhBMP-2 on regeneration of alveolar bone and periodontal attachment used in conjunction with a space-providing ePTFE device. Methods: Routine, critical-size, 5-6 mm, supra-alveolar, periodontal defects were created around the third and fourth mandibular premolar teeth in four young adult Hound Labrador mongrel dogs. rhBMP-2 (0.2 mg/ml) in an absorbable collagen sponge (rhBMP-2/ACS) or buffer/ACS (control) implants were randomly assigned to be placed around the premolar teeth in the left and right jaw quadrants in subsequent animals. Space-providing ePTFE devices with 300-μm laser-drilled pores, 0.8 mm apart, were used to cover the rhBMP-2 and control implants. The gingival flaps were advanced for primary wound closure. The animals were euthanized at 8 weeks postsurgery for histologic and histometric analyses. Results: Bone regeneration and ankylosis were significantly increased in jaw quadrants receiving rhBMP-2/ACS compared to control (bone height 4.8 ± 0.3 versus 2.0 ± 0.2 mm, p = 0.001; bone area 10.9 ± 1.3 versus 1.4 ± 0.1 mm2; p = 0.009, and ankylosis 2.2 ± 0.2 versus 0.04 ± 0.7 mm; p = 0.01). No differences between groups were found for cementum regeneration and root resorption. Conclusions: rhBMP-2 significantly enhances regeneration of alveolar bone in conjunction with a space-providing, macroporous ePTFE device for GTR.
AB - Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) has been shown to support the regeneration of alveolar bone and periodontal attachment in surgically created periodontal defects and in defects with a history of dental plaque and calculus exposure. Periodontal regeneration has also been shown following guided tissue regeneration using space-providing expanded polytetrafluoroethylene (ePTFE) devices. The objective of this study was to evaluate the influence of rhBMP-2 on regeneration of alveolar bone and periodontal attachment used in conjunction with a space-providing ePTFE device. Methods: Routine, critical-size, 5-6 mm, supra-alveolar, periodontal defects were created around the third and fourth mandibular premolar teeth in four young adult Hound Labrador mongrel dogs. rhBMP-2 (0.2 mg/ml) in an absorbable collagen sponge (rhBMP-2/ACS) or buffer/ACS (control) implants were randomly assigned to be placed around the premolar teeth in the left and right jaw quadrants in subsequent animals. Space-providing ePTFE devices with 300-μm laser-drilled pores, 0.8 mm apart, were used to cover the rhBMP-2 and control implants. The gingival flaps were advanced for primary wound closure. The animals were euthanized at 8 weeks postsurgery for histologic and histometric analyses. Results: Bone regeneration and ankylosis were significantly increased in jaw quadrants receiving rhBMP-2/ACS compared to control (bone height 4.8 ± 0.3 versus 2.0 ± 0.2 mm, p = 0.001; bone area 10.9 ± 1.3 versus 1.4 ± 0.1 mm2; p = 0.009, and ankylosis 2.2 ± 0.2 versus 0.04 ± 0.7 mm; p = 0.01). No differences between groups were found for cementum regeneration and root resorption. Conclusions: rhBMP-2 significantly enhances regeneration of alveolar bone in conjunction with a space-providing, macroporous ePTFE device for GTR.
KW - Periodontal regeneration
KW - Seroma
KW - Tissue engineering
KW - Wound healing
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U2 - 10.1034/j.1600-051X.2003.00363.x
DO - 10.1034/j.1600-051X.2003.00363.x
M3 - Article
C2 - 12887339
AN - SCOPUS:0142093240
SN - 0303-6979
VL - 30
SP - 705
EP - 714
JO - Journal of Clinical Periodontology
JF - Journal of Clinical Periodontology
IS - 8
ER -