Periodontal repair in dogs: Space-providing ePTFE devices increase rhBMP-2/ACS-induced bone formation

Ulf M E Wikesjö, Andreas V. Xiropaidis, Robert C. Thomson, Alonzo D. Cook, Knut A. Selvig, W. Ross Hardwick

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) technologies have been shown to enhance alveolar bone formation significantly. Biomaterial (carrier) limitations, however, have restricted their biologic potential for indications where compressive forces may limit the volume of bone formed. The objective of this proof-of-principle study was to evaluate the potential of a space-providing, macroporous ePTFE device to define rhBMP-2-induced alveolar bone formation using a discriminating onlay defect model. Methods: Routine, critical size, 5-6 mm, supra-alveolar, periodontal defects were created around the third and fourth mandibular premolar teeth in four young adult Hound Labrador mongrel dogs. All jaw quadrants received rhBMP-2 (0.4 mg) in an absorbable collagen sponge (ACS) carrier. Contralateral jaw quadrants in subsequent animals were randomly assigned to receive additionally the dome-shaped, macroporous ePTFE device over the rhBMP-2/ACS implant or no additional treatment. The gingival flaps were advanced to cover the ePTFE device and teeth, and sutured. Animals were scheduled for euthanasia to provide for histologic observations of healing at 8 weeks postsurgery. Results: Healing was uneventful without device exposures. New bone formation averaged (± SD) 4.7 ± 0.2 mm (98%) and 4.5 ± 0.4 mm (94%) of the defect height, respectively, for jaw quadrants receiving rhBMP-2/ACS with the ePTFE device or rhBMP-2/ACS alone (p > 0.05). In contrast, the regenerated bone area was significantly enhanced in jaw quadrants receiving rhBMP-2/ACS with the ePTFE device compared to rhBMP-2/ACS alone (9.3 ± 2.7 versus 5.1 ± 1.1 mm2; p < 0.05). Cementum formation was similar for both treatment groups. Ankylosis compromised periodontal regeneration in all sites. Conclusions: The results suggest that the novel space-providing, macroporous ePTFE device appears suitable as a template to define rhBMP-2/ACS-induced alveolar bone formation.

Original languageEnglish (US)
Pages (from-to)715-725
Number of pages11
JournalJournal of Clinical Periodontology
Issue number8
StatePublished - Aug 2003


  • Periodontal regeneration
  • Seroma
  • Tissue engineering
  • Wound healing

ASJC Scopus subject areas

  • Periodontics


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