piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells

Sareina Chiung Yuan Wu; Yaa Jyuhn James Meir; Craig J. Coates; Alfred M. Handler; Pawel Pelczar; Stefan Moisyadi; Joseph M. Kaminski

doi:10.1073/pnas.0606979103

piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells

Sareina Chiung Yuan Wu, Yaa Jyuhn James Meir, Craig J. Coates, Alfred M. Handler, Pawel Pelczar, Stefan Moisyadi, Joseph M. Kaminski

Research output: Contribution to journal › Article › peer-review

279 Scopus citations

Abstract

A nonviral vector for highly efficient site-specific integration would be desirable for many applications in transgenesis, including gene therapy. In this study we directly compared the genomic integration efficiencies of piggyBac, hyperactive Sleeping Beauty (SB11), Tol2, and Mos1 in four mammalian cell lines. piggyBac demonstrated significantly higher transposition activity in all cell lines whereas Mos1 had no activity. Furthermore, piggyBac transposase coupled to the GAL4 DNA-binding domain retains transposition activity whereas similarly manipulated gene products of Tol2 and SB11 were inactive. The high transposition activity of piggyBac and the flexibility for molecular modification of its transposase suggest the possibility of using it routinely for mammalian transgenesis.

Original language	English (US)
Pages (from-to)	15008-15013
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	103
Issue number	41
DOIs	https://doi.org/10.1073/pnas.0606979103
State	Published - Oct 10 2006
Externally published	Yes

Keywords

Cancer
Gene therapy
Mutagenesis
Site-specific
Transposase

ASJC Scopus subject areas

General

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1073/pnas.0606979103

Cite this

Wu, S. C. Y., Meir, Y. J. J., Coates, C. J., Handler, A. M., Pelczar, P., Moisyadi, S., & Kaminski, J. M. (2006). piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells. Proceedings of the National Academy of Sciences of the United States of America, 103(41), 15008-15013. https://doi.org/10.1073/pnas.0606979103

piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells. / Wu, Sareina Chiung Yuan; Meir, Yaa Jyuhn James; Coates, Craig J. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 41, 10.10.2006, p. 15008-15013.

Research output: Contribution to journal › Article › peer-review

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title = "piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells",

abstract = "A nonviral vector for highly efficient site-specific integration would be desirable for many applications in transgenesis, including gene therapy. In this study we directly compared the genomic integration efficiencies of piggyBac, hyperactive Sleeping Beauty (SB11), Tol2, and Mos1 in four mammalian cell lines. piggyBac demonstrated significantly higher transposition activity in all cell lines whereas Mos1 had no activity. Furthermore, piggyBac transposase coupled to the GAL4 DNA-binding domain retains transposition activity whereas similarly manipulated gene products of Tol2 and SB11 were inactive. The high transposition activity of piggyBac and the flexibility for molecular modification of its transposase suggest the possibility of using it routinely for mammalian transgenesis.",

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AU - Coates, Craig J.

AU - Handler, Alfred M.

AU - Pelczar, Pawel

AU - Moisyadi, Stefan

AU - Kaminski, Joseph M.

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AB - A nonviral vector for highly efficient site-specific integration would be desirable for many applications in transgenesis, including gene therapy. In this study we directly compared the genomic integration efficiencies of piggyBac, hyperactive Sleeping Beauty (SB11), Tol2, and Mos1 in four mammalian cell lines. piggyBac demonstrated significantly higher transposition activity in all cell lines whereas Mos1 had no activity. Furthermore, piggyBac transposase coupled to the GAL4 DNA-binding domain retains transposition activity whereas similarly manipulated gene products of Tol2 and SB11 were inactive. The high transposition activity of piggyBac and the flexibility for molecular modification of its transposase suggest the possibility of using it routinely for mammalian transgenesis.

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piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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