TY - JOUR
T1 - Plasma BDNF and PDGF-AA levels are associated with high TCD velocity and stroke in children with sickle cell anemia
AU - Hyacinth, Hyacinth I.
AU - Gee, Beatrice E.
AU - Adamkiewicz, Thomas V.
AU - Adams, Robert J.
AU - Kutlar, Abdullah
AU - Stiles, Jonathan K.
AU - Hibbert, Jacqueline M.
N1 - Funding Information:
This work was support in part by grants from the National Institutes of Health NIH-RCMI-RR033062, NIH-NHLBI-R21HL92358, NIH-FIC-1R90-HG004151-01, NIH-ACTSI-UL-1RR025008 and the Cooperative Agreements with the National Heart, Lung, and Blood Institute (U10 HL 52193 and U10 HL 52016). Also, the authors wish to acknowledge Ms. Jane Chu of the proteomics core for logistical support during sample analysis.
PY - 2012/10
Y1 - 2012/10
N2 - Sickle cell anemia (SCA) associated cerebrovascular disease includes vascular remodeling, abnormal cerebral blood flow (CBF) and infarction. We studied the relationships between plasma brain derived neurotropic factor (BDNF), platelet derived growth factors (PDGF-AA and -AB/BB) and high trans-cranial Doppler (TCD) velocity, an indication of CBF velocity. Baseline plasma samples from 39 children (19 SCA with abnormal/high TCD [SATCD], 13 SCA with normal TCD [SNTCD] and 7 healthy non-SCA), were assayed for BDNF, PDGF-AA and -AB/BB plus 11 other cytokines. The sensitivity, specificity and usefulness of these biomarkers for stroke prediction was investigated. All subject groups were of similar age and gender distribution. Mean BDNF was significantly higher among SATCD than SNTCD (p=0.004) as was mean PDGF-AA (p=0.001). Similarly, mean PDGF-AA was higher among SCA subjects who developed stroke than those who did not (p=0.012). Elevated BDNF and PDGF-AA were good predictors of the presence of abnormally high CBF velocity and were both associated with severity of anemia. Elevated PDGF-AA predicted risk for stroke development. Stroke incidence and high TCD velocity were associated with elevated BDNF and PDGF-AA. These findings suggest a role for BDNF and PDGF-AA in the patho-physiological mechanism of cerebrovascular disease in SCA.
AB - Sickle cell anemia (SCA) associated cerebrovascular disease includes vascular remodeling, abnormal cerebral blood flow (CBF) and infarction. We studied the relationships between plasma brain derived neurotropic factor (BDNF), platelet derived growth factors (PDGF-AA and -AB/BB) and high trans-cranial Doppler (TCD) velocity, an indication of CBF velocity. Baseline plasma samples from 39 children (19 SCA with abnormal/high TCD [SATCD], 13 SCA with normal TCD [SNTCD] and 7 healthy non-SCA), were assayed for BDNF, PDGF-AA and -AB/BB plus 11 other cytokines. The sensitivity, specificity and usefulness of these biomarkers for stroke prediction was investigated. All subject groups were of similar age and gender distribution. Mean BDNF was significantly higher among SATCD than SNTCD (p=0.004) as was mean PDGF-AA (p=0.001). Similarly, mean PDGF-AA was higher among SCA subjects who developed stroke than those who did not (p=0.012). Elevated BDNF and PDGF-AA were good predictors of the presence of abnormally high CBF velocity and were both associated with severity of anemia. Elevated PDGF-AA predicted risk for stroke development. Stroke incidence and high TCD velocity were associated with elevated BDNF and PDGF-AA. These findings suggest a role for BDNF and PDGF-AA in the patho-physiological mechanism of cerebrovascular disease in SCA.
KW - Biomarkers
KW - Cerebral ischemia
KW - Sickle cell anemia
KW - Stroke
KW - Trans-cranial Doppler
UR - http://www.scopus.com/inward/record.url?scp=84865416509&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865416509&partnerID=8YFLogxK
U2 - 10.1016/j.cyto.2012.05.017
DO - 10.1016/j.cyto.2012.05.017
M3 - Article
C2 - 22704695
AN - SCOPUS:84865416509
SN - 1043-4666
VL - 60
SP - 302
EP - 308
JO - Cytokine
JF - Cytokine
IS - 1
ER -