Plasma lactate levels increase during hyperinsulinemic euglycemic clamp and oral glucose tolerance test

Feven Berhane, Alemu Fite, Nour Daboul, Wissam Al-Janabi, Zaher Msallaty, Michael Caruso, Monique K. Lewis, Zhengping Yi, Michael P. Diamond, Abdul Badi Abou-Samra, Berhane Seyoum

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of this study was to determine the pattern of circulating lactate levels during oral glucose tolerance test (OGTT) and hyperinsulinemic euglycemic clamp (HIEC) study in normal nondiabetic subjects. Lactate and glycerol were determined every 30 minutes during OGTT and HIEC on 22 participants. Lactate progressively increased throughout the HIEC study period (P < 0.001). Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P < 0.05). This trend also continued throughout the OGTT. In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001). In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.

Original languageEnglish (US)
Article number102054
JournalJournal of Diabetes Research
StatePublished - 2015

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology


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