@article{e803d471a3414f3e82a3db165ebba06a,
title = "Polyketide Ring Expansion Mediated by a Thioesterase, Chain Elongation and Cyclization Domain, in Azinomycin Biosynthesis: Characterization of AziB and AziG",
abstract = "The azinomycins are a family of potent antitumor agents with the ability to form interstrand cross-links with DNA. This study reports on the unusual biosynthetic formation of the 5-methyl naphthoate moiety, which is essential for effective DNA association. While sequence analysis predicts that the polyketide synthase (AziB) catalyzes the formation of this naphthoate, 2-methylbenzoic acid, a truncated single-ring product, is formed instead. We demonstrate that the thioesterase (AziG) acts as a chain elongation and cyclization (CEC) domain and is required for the additional two rounds of chain extension to form the expected product.",
author = "Shogo Mori and Dinesh Simkhada and Huitu Zhang and Erb, {Megan S.} and Yang Zhang and Howard Williams and Dmytro Fedoseyenko and Russell, {William K.} and Doyong Kim and Nathan Fleer and Ealick, {Steven E.} and Watanabe, {Coran M.H.}",
note = "Funding Information: We are grateful to the National Science Foundation (CHE- 1308151, C.M.H.W.; CHE-1308222, S.E.E.), the National Institutes of Health (T32 GM008500, M.S.E.), and the Welch Foundation (A-1828, C.M.H.W.) for financial support. Beam time was provided by the Advanced Photon Source on the Northeastern Collaborative Access Team beamlines (P41 GM103403 and S10 RR029205 from the National Institutes of Health). Use of the Advanced Photon Source is supported by the U.S. Department of Energy, Office of Basic Energy Sciences, under Contract No. DE-AC02-06CH11357. Use of the Cornell High Energy Synchrotron Source is supported by the National Science Foundation (DMR-0936384) and National Institutes of Health (P41 GM103485). The authors thank Benjamin Philmus, Yiquan Liu, and Yindrila Chakrabarty for LC/MS analyses and Tadhg P. Begley for helpful discussions. Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",
year = "2016",
month = feb,
day = "2",
doi = "10.1021/acs.biochem.5b01050",
language = "English (US)",
volume = "55",
pages = "704--714",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "4",
}