TY - JOUR
T1 - Porphyromonas gingivalis endotoxin affinity for dental ceramics
AU - Robinson, Fonda G.
AU - Knoernschild, Kent L.
AU - Sterrett, John D.
AU - Tompkins, Geoffrey R.
N1 - Funding Information:
T he development of dental plaque around gingival margins of teeth has been associated with the clinical onset of gingivitis. is2 The shift from periodontal health to gingivitis involves an increase in both the quantity and types of crevicular gram-negative bacteria, which comprise 5% to 31% of the microbial flora.le5 On the other hand, gram-negative bacteria of subgingival periodontitis sites have been found to make up 22% to 89% of the microorganisms.5-7 One distinguishing feature of gram-negative bacteria is the cell envelope, which contains lipopolysaccharide (LPS) and is commonly referred to as endotoxin. This LPS can pass through oral-sulcular epitheliums and has been associated with cementum, loosely adherent subgingival plaque, and crevicular fluid.g, lo LPS can elicit a variety of biologic reactions. The lipid A portion of the molecule activates the classic pathway of the complement system, whereas the core sugar portion activates the complement system via the alternate pathway.ll LPS also stimulates macrophage release of collagenase,12 induces osteoclastic bone resorption,13-17 and stimulates prostaglandin release. l8 Furthermore, LPS is cytotoxic for Supported in part by an American Academy of Fixed Prosthodon-tics Tylman research grant. 1994 Tylman Award winner. 1995 Arthur R. Frechette Research Award winner. aAssistant Professor, Department of Oral Rehabilitation. bAssistant Professor, Departments of Oral Rehabilitation and Oral Biology. “Associate Professor, Department of Periodontics. dAssistant Professor, Department of Oral Biology. Copyright 0 1996 by The Editorial Council of THE JOURNAL OF PROSTHETIC DENTISTRY. 0022-3913/96/$5.00 + 0. 10/l/68923 both epithelial cellslg and fibroblasts.20 Such mechanisms are thought to play a major role in tissue destruction seen in periodontal disease.
PY - 1996
Y1 - 1996
N2 - This study evaluated the effects of chemical composition, surface treatment, and initial exposure dose on Porphyromonas gingivalis lipopolysaccharide adherence to and elution from dental ceramics. Lipopolysaccharide, commonly known as endotoxin, can initiate a variety of biologic responses. Opaque, body, and Dicor ceramic disks were individually exposed to 250, 1000, or 2500 EU/ml 3 H-lipopolysaccharide and incubated for 24 hours at 37° C. Disks were then transferred to fresh lipopolysaccharide-free water and incubated for up to 96 hours to evaluate elution. Mean initial lipopolysaccharide adherence ranged from 0.397 ± 0.048 EU/mm2 to 5.056 ± 0.117 EU/mm2. Greater initial exposure levels resulted in greater adherence, and at higher lipopolysaccharide exposure levels, lipopolysaccharide adherence differences were based on ceramic type. Mean lipopolysaccharide elution levels ranged from 0.063 ± 0.02 EU/mm2 to 0.00 EU/mm2 at 96 hours for all groups. Greater initial adherence resulted in greater elution. Ceramic type did not affect elution. Surface finish affected elution at the 2500 EU exposure level. The affinity of lipopolysaccharide for dental ceramics could contribute to a periodontal inflammatory process.
AB - This study evaluated the effects of chemical composition, surface treatment, and initial exposure dose on Porphyromonas gingivalis lipopolysaccharide adherence to and elution from dental ceramics. Lipopolysaccharide, commonly known as endotoxin, can initiate a variety of biologic responses. Opaque, body, and Dicor ceramic disks were individually exposed to 250, 1000, or 2500 EU/ml 3 H-lipopolysaccharide and incubated for 24 hours at 37° C. Disks were then transferred to fresh lipopolysaccharide-free water and incubated for up to 96 hours to evaluate elution. Mean initial lipopolysaccharide adherence ranged from 0.397 ± 0.048 EU/mm2 to 5.056 ± 0.117 EU/mm2. Greater initial exposure levels resulted in greater adherence, and at higher lipopolysaccharide exposure levels, lipopolysaccharide adherence differences were based on ceramic type. Mean lipopolysaccharide elution levels ranged from 0.063 ± 0.02 EU/mm2 to 0.00 EU/mm2 at 96 hours for all groups. Greater initial adherence resulted in greater elution. Ceramic type did not affect elution. Surface finish affected elution at the 2500 EU exposure level. The affinity of lipopolysaccharide for dental ceramics could contribute to a periodontal inflammatory process.
UR - http://www.scopus.com/inward/record.url?scp=0030075747&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030075747&partnerID=8YFLogxK
U2 - 10.1016/S0022-3913(96)90102-X
DO - 10.1016/S0022-3913(96)90102-X
M3 - Article
C2 - 8667283
AN - SCOPUS:0030075747
SN - 0022-3913
VL - 75
SP - 217
EP - 227
JO - Journal of Prosthetic Dentistry
JF - Journal of Prosthetic Dentistry
IS - 2
ER -