Potassium currents from isolated frog lens epithelial cells

Kim Cooper; Mitchell Watsky; James Rae

doi:10.1016/0014-4835(92)90012-H

Potassium currents from isolated frog lens epithelial cells

Kim Cooper
, Mitchell Watsky
, James Rae

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Using the perforated patch version of whole-cell recording, we have measurd currents from isolated frog lens epithelial cells. Three types of currents were seen. A time-independent outwardly rectifying potassium current was identified that sets the resting voltage. This potassium current differs significantly from any of the potassium currents recorded with the whole-cell technique in mammalian lens epithelial cells. In addition to the potassium current, the two other currents present were both outwardly rectifying: one was time-independent while the other showed distinct activation.

Original language	English (US)
Pages (from-to)	861-868
Number of pages	8
Journal	Experimental eye research
Volume	55
Issue number	6
DOIs	https://doi.org/10.1016/0014-4835(92)90012-H
State	Published - Dec 1992
Externally published	Yes

Keywords

K current
frog lens epithelium
ion transport
patch clamp
resting voltage
voltage clamp

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.1016/0014-4835(92)90012-H

Cite this

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title = "Potassium currents from isolated frog lens epithelial cells",

abstract = "Using the perforated patch version of whole-cell recording, we have measurd currents from isolated frog lens epithelial cells. Three types of currents were seen. A time-independent outwardly rectifying potassium current was identified that sets the resting voltage. This potassium current differs significantly from any of the potassium currents recorded with the whole-cell technique in mammalian lens epithelial cells. In addition to the potassium current, the two other currents present were both outwardly rectifying: one was time-independent while the other showed distinct activation.",

keywords = "K current, frog lens epithelium, ion transport, patch clamp, resting voltage, voltage clamp",

author = "Kim Cooper and Mitchell Watsky and James Rae",

note = "Funding Information: The authorsw ould like to acknowledgeE rika Wohlfielf or expert technical assistancein preparing this manuscript. This work was supportedb y NIH grants EYO3282. EY06005, EY09636 and an unrestricted award from Researcht o PreventB lindness.",

year = "1992",

month = dec,

doi = "10.1016/0014-4835(92)90012-H",

language = "English (US)",

volume = "55",

pages = "861--868",

journal = "Experimental eye research",

issn = "0014-4835",

publisher = "Academic Press",

number = "6",

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TY - JOUR

T1 - Potassium currents from isolated frog lens epithelial cells

AU - Cooper, Kim

AU - Watsky, Mitchell

AU - Rae, James

N1 - Funding Information: The authorsw ould like to acknowledgeE rika Wohlfielf or expert technical assistancein preparing this manuscript. This work was supportedb y NIH grants EYO3282. EY06005, EY09636 and an unrestricted award from Researcht o PreventB lindness.

PY - 1992/12

Y1 - 1992/12

N2 - Using the perforated patch version of whole-cell recording, we have measurd currents from isolated frog lens epithelial cells. Three types of currents were seen. A time-independent outwardly rectifying potassium current was identified that sets the resting voltage. This potassium current differs significantly from any of the potassium currents recorded with the whole-cell technique in mammalian lens epithelial cells. In addition to the potassium current, the two other currents present were both outwardly rectifying: one was time-independent while the other showed distinct activation.

AB - Using the perforated patch version of whole-cell recording, we have measurd currents from isolated frog lens epithelial cells. Three types of currents were seen. A time-independent outwardly rectifying potassium current was identified that sets the resting voltage. This potassium current differs significantly from any of the potassium currents recorded with the whole-cell technique in mammalian lens epithelial cells. In addition to the potassium current, the two other currents present were both outwardly rectifying: one was time-independent while the other showed distinct activation.

KW - K current

KW - frog lens epithelium

KW - ion transport

KW - patch clamp

KW - resting voltage

KW - voltage clamp

UR - https://www.scopus.com/pages/publications/0027043215

UR - https://www.scopus.com/pages/publications/0027043215#tab=citedBy

U2 - 10.1016/0014-4835(92)90012-H

DO - 10.1016/0014-4835(92)90012-H

M3 - Article

C2 - 1486942

AN - SCOPUS:0027043215

SN - 0014-4835

VL - 55

SP - 861

EP - 868

JO - Experimental eye research

JF - Experimental eye research

IS - 6

ER -

Potassium currents from isolated frog lens epithelial cells

Abstract

Keywords

ASJC Scopus subject areas

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