Preparation of whole bone marrow for mass cytometry analysis of neutrophil-lineage cells

Yanfang Peipei Zhu, Lindsey Padgett, Huy Q. Dinh, Paola Marcovecchio, Runpei Wu, Denise Hinz, Cheryl Kim, Catherine C. Hedrick

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


In this article, we present a protocol that is optimized to preserve neutrophil-lineage cells in fresh BM for whole BM CyTOF analysis. We utilized a myeloid-biased 39-antibody CyTOF panel to evaluate the hematopoietic system with a focus on the neutrophil-lineage cells by using this protocol. The CyTOF result was analyzed with an open-resource dimensional reduction algorithm, viSNE, and the data was presented to demonstrate the outcome of this protocol. We have discovered new neutrophil-lineage cell populations based on this protocol. This protocol of fresh whole BM preparation may be used for 1), CyTOF analysis to discover unidentified cell populations from whole BM, 2), investigating whole BM defects for patients with blood disorders such as leukemia, 3), assisting optimization of fluorescence-activated flow cytometry protocols that utilize fresh whole BM.

Original languageEnglish (US)
Article numbere59617
JournalJournal of Visualized Experiments
Issue number148
StatePublished - Jun 2019
Externally publishedYes


  • BM
  • Bone Marrow
  • CyTOF
  • Flow Cytometry
  • Immunology and Infection
  • Issue 148
  • Mass Cytometry
  • Neutrophil-lineage Cells
  • ViSNE

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


Dive into the research topics of 'Preparation of whole bone marrow for mass cytometry analysis of neutrophil-lineage cells'. Together they form a unique fingerprint.

Cite this