Prevention of ischemia-reperfusion lung injury by sulfated Lewisa pentasaccharide

Jean Reignier, Hassan Sellak, Rémy Lemoine, André Lubineau, Guy Michel Mazmanian, Hélène Detruit, Alain Chapelier, Philippe Hervé

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Inhibition of polymorphonuclear neutrophil (PMN) adhesion to the pulmonary endothelium attenuates ischemia-reperfusion (I/R) lung injury. We hypothesized that 3'-sulfated Lewisa (SuLa), a potent ligand for the selectin adhesion molecules, may have a beneficial effect on I/R lung injury, as measured by the filtration coefficient (K(fe)), and reduce pulmonary sequestration of PMN as assessed by the lung myeloperoxidase (MPO) activity. Blood-perfused rat lungs were subjected to 30 min of perfusion, 60 min of warm ischemia, and 90 min of reperfusion after treatment with either SuLa (200 μg) or saline. Effects of SuLa on PMN adhesion to cultured human umbilical vein endothelial cells (HUVEC) stimulated with tumor necrosis factor-α and calcium ionophore were also investigated. Compared with preischemia conditions, I/R induced a significant increase in K(fe), which was attenuated with SuLa (80 ± 8 vs. 30 ± 5%; P < 0.001). SuLa reduced lung MPO and PMN adhesion to stimulated HUVEC. These results indicate that SuLa reduces I/R-induced lung injury and PMN accumulation in lung. This protective effect might be related to inhibition of PMN adhesion to endothelial cells.

Original languageEnglish (US)
Pages (from-to)1058-1063
Number of pages6
JournalJournal of Applied Physiology
Issue number4
StatePublished - Apr 1997


  • endothelium
  • neutrophils
  • rat lung
  • selectins

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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