@article{86c78ccbbf684b2596868f4e19af7a24,
title = "Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3",
abstract = "Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, 3 monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal origins but neither galectin-2 nor galectin-3. Western-blotting analysis of soluble human brain extracts after 2D gel electrophoresis revealed only the two most acidic isoforms of galectin-1. The ability of this mAb to bind galectin-1-asialofetuin complexes indicates that its epitope is not localized in galacarbohydrate recognition domain of galectin-1. This particularity induces with efficiency its monospecificity.",
keywords = "Galectin/monoclonal antibody/carbohydrate recognition domain",
author = "Cornillot, {Jean Denis} and Michel Pontet and Catherine Dupuy and Ahmed Chadli and Michel Caron and Raymonde Joubert-Caron and Philippe Bourin and Dominique Bladier",
note = "Funding Information: This work has been supported by grants from the Minist{\`e}re de l{\textquoteright}Education Nationale de l{\textquoteright}Enseignement Sup{\'e}rieur et de la Recherche. J. D. Cornillot is an Allocataire de Recherche (Minist{\`e}re de l{\textquoteright}Education Nationale de l{\textquoteright}Enseignement Sup{\'e}rieur et de la Recherche). We thank Dr. F.-T. Liu (La Jolla Institute for Allergy and Immunology, San Diego, CA), Dr. A.-P. S{\`e}ve (INSERM U 180, Paris, France), and Dr. M. A. Gitt and Dr. H. Leffler (Department of Psychiatry and Langley Porter Psychiatric Institute, University of California, San Francisco, CA) for the gift of human and mouse galectin-3 and galectin-2, respectively, and also Dr L. Astic (Laboratoire de Physiologie Neurosensorielle, Universit{\'e} Claude Bernard, Lyon-1) and Dr. M. F{\`e}vre-Montange (Laboratoire de Neurobiologie Exp{\'e}rimentale et Physiopathologie, Facult{\'e} de M{\'e}decine R. Laennec, Universit{\'e} Lyon-1) for their assistance in immunochemistry and in situ hybridization, respectively.",
year = "1998",
month = may,
doi = "10.1093/glycob/8.5.425",
language = "English (US)",
volume = "8",
pages = "425--432",
journal = "Glycobiology",
issn = "0959-6658",
publisher = "Oxford University Press",
number = "5",
}