Progesterone stimulates in vitro release of prolactin and thyrotropin involving cAMP production in rat pituitary

Shiow Chwen Tsai, Chien Chen Lu, Chin Pang Lau, Guey Shyang Hwang, Hsien Yang Lee, Shing Liang Chen, Seng Wong Huang, Hsi Chang Shih, Yen Hao Chen, Yu Chung Chiao, Shyi Wu Wang, Paulus S. Wang

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Effects of progesterone administration in vivo or in vitro on the release of pituitary prolactin (PRL) and thyroid stimulating hormone (TSH) in vitro, as well as the generation of pituitary cyclic adenosine 3', 5'-monophosphate (cAMP) were studied in ovariectomized (Ovx) rats. Ovx rats were either decapitated or injected subcutaneously with progesterone, or oil for 3 days before decapitation. The anterior pituitary glands (APs) of progesterone treated Ovx rats were incubated with Locke's medium containing thyrotropin-releasing hormone (TRH, 10 nM) and/or 3-isobutyl-1-methylxanthine (IBMX, 1 mM) at 37 °C for 30 min. The APs of untreated Ovx rats were incubated in vitro with IBMX and progesterone (0, 0.1, 1, 10 nM). Progesterone increased spontaneous and TRH-induced release of PRL and TSH in vitro. Administration of progesterone, either in vivo or in vitro, increased the production of pituitary cAMP following incubation of IBMX. These results suggest that progesterone increases the release of PRL and TSH through a mechanism associated with an increase of cAMP production in rat APs.

Original languageEnglish (US)
Pages (from-to)245-251
Number of pages7
JournalChinese Journal of Physiology
Issue number4
StatePublished - 1996


  • PRL
  • TSH
  • anterior pituitary
  • cAMP
  • progesterone
  • rat

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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