Protection from septic peritonitis by rapid neutrophil recruitment through omental high endothelial venules

Konrad Buscher, Huiyu Wang, Xueli Zhang, Paul Striewski, Benedikt Wirth, Gurpanna Saggu, Stefan Lütke-Enking, Tanya N. Mayadas, Klaus Ley, Lydia Sorokin, Jian Song

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Acute peritonitis is a frequent medical condition that can trigger severe sepsis as a life-threatening complication. Neutrophils are first-responders in infection but recruitment mechanisms to the abdominal cavity remain poorly defined. Here, we demonstrate that high endothelial venules (HEVs) of the greater omentum constitute a main entry pathway in TNFα-, Escherichia coli (E. coli)-and caecal ligation and puncture-induced models of inflammation. Neutrophil transmigration across HEVs is faster than across conventional postcapillary venules and requires a unique set of adhesion receptors including peripheral node addressin, E-, L-selectin and Mac-1 but not P-selectin or LFA-1. Omental milky spots readily concentrate intra-abdominal E. coli where macrophages and recruited neutrophils collaborate in phagocytosis and killing. Inhibition of the omental neutrophil response exacerbates septic progression of peritonitis. This data identifies HEVs as a clinically relevant vascular recruitment site for neutrophils in acute peritonitis that is indispensable for host defence against early systemic bacterial spread and sepsis.

Original languageEnglish (US)
Article number10828
JournalNature communications
Volume7
DOIs
StatePublished - Mar 4 2016
Externally publishedYes

ASJC Scopus subject areas

  • General Chemistry
  • General Biochemistry, Genetics and Molecular Biology
  • General Physics and Astronomy

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