TY - JOUR
T1 - Protective effect of reduced glutathione on the liver injury induced by acute omethoate poisoning
AU - Lu, Lina
AU - Wang, Xiuli
AU - Lang, Liwei
AU - Fu, Fenghua
N1 - Funding Information:
This study was supported by grants from the Health Bureau of Shandong (No. 98CAICFA2 ), the Educational Bureau of Shandong (No. J01k11 ). The authors would like to thank Dr. Yan Zhang for her technical assistance.
PY - 2010/11
Y1 - 2010/11
N2 - Omethoate is an organophosphate insecticide with high toxicity. The aim of this study was to investigate the protective effect of exogenous reduced glutathione (GSH) on omethoate-induced liver injury. Sprague-Dawley rats were randomly divided into three groups: control, OM (omethoate poisoning), and OM. +. GSH (omethoate poisoning treated with GSH). The activities of acetylcholinesterase (AChE), aspartate aminotransferase (AST), alanine aminotransferase (ALT) in plasma, free organophosphate (FOP) in the liver were determined, and the histopathological changes in the liver were observed. Furthermore, TNF-α and NO in liver homogenate were assayed. The results showed that AChE activity was significantly inhibited by omethoate, but was not altered by GSH treatment. GSH was able to prevent hepatocellular edema and fatty degeneration, decrease liver FOP, attenuate the increased AST and ALT activity, and decline the increase of TNF-α and NO induced by omethoate. These results indicate GSH can attenuate liver injury, and suggest that GSH may be administered to protect the organ from injury in patients with acute organophosphate poisoning.
AB - Omethoate is an organophosphate insecticide with high toxicity. The aim of this study was to investigate the protective effect of exogenous reduced glutathione (GSH) on omethoate-induced liver injury. Sprague-Dawley rats were randomly divided into three groups: control, OM (omethoate poisoning), and OM. +. GSH (omethoate poisoning treated with GSH). The activities of acetylcholinesterase (AChE), aspartate aminotransferase (AST), alanine aminotransferase (ALT) in plasma, free organophosphate (FOP) in the liver were determined, and the histopathological changes in the liver were observed. Furthermore, TNF-α and NO in liver homogenate were assayed. The results showed that AChE activity was significantly inhibited by omethoate, but was not altered by GSH treatment. GSH was able to prevent hepatocellular edema and fatty degeneration, decrease liver FOP, attenuate the increased AST and ALT activity, and decline the increase of TNF-α and NO induced by omethoate. These results indicate GSH can attenuate liver injury, and suggest that GSH may be administered to protect the organ from injury in patients with acute organophosphate poisoning.
KW - Acetylcholinesterase
KW - Acute poisoning
KW - Liver injury
KW - Omethoate
KW - Organophosphate
KW - Reduced glutathione
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U2 - 10.1016/j.etap.2010.08.001
DO - 10.1016/j.etap.2010.08.001
M3 - Article
AN - SCOPUS:77957364464
SN - 1382-6689
VL - 30
SP - 279
EP - 283
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
IS - 3
ER -