Proteomic studies of the intrinsically unstructured mammalian proteome

Charles A. Galea; Vishwajeeth R. Pagala; John C. Obenauer; Cheon Gil Park; Clive A. Slaughter; Richard W. Kriwacki

doi:10.1021/pr060328c

Proteomic studies of the intrinsically unstructured mammalian proteome

Charles A. Galea, Vishwajeeth R. Pagala, John C. Obenauer, Cheon Gil Park, Clive A. Slaughter, Richard W. Kriwacki

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

Intrinsically unstructured proteins (IUPs) represent an important class of proteins primarily involved in cellular signaling and regulation. The aim of this study was to develop methodology for the enrichment and identification of IUPs. We show that heat treatment of NIH3T3 mouse fibroblast cell extracts at 98 °C selects for IUPs. The majority of these IUPs were cytosolic or nuclear proteins involved in cell signaling or regulation. These studies represent the first large-scale experimental investigation of the intrinsically unstructured mammalian proteome.

Original language	English (US)
Pages (from-to)	2839-2848
Number of pages	10
Journal	Journal of Proteome Research
Volume	5
Issue number	10
DOIs	https://doi.org/10.1021/pr060328c
State	Published - Oct 2006
Externally published	Yes

Keywords

Disordered proteins
Heat denaturation
Intrinsically unstructured proteins
Mammalian proteome
Proteomics

ASJC Scopus subject areas

Biochemistry
General Chemistry

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10.1021/pr060328c

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abstract = "Intrinsically unstructured proteins (IUPs) represent an important class of proteins primarily involved in cellular signaling and regulation. The aim of this study was to develop methodology for the enrichment and identification of IUPs. We show that heat treatment of NIH3T3 mouse fibroblast cell extracts at 98 °C selects for IUPs. The majority of these IUPs were cytosolic or nuclear proteins involved in cell signaling or regulation. These studies represent the first large-scale experimental investigation of the intrinsically unstructured mammalian proteome.",

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AU - Pagala, Vishwajeeth R.

AU - Obenauer, John C.

AU - Park, Cheon Gil

AU - Slaughter, Clive A.

AU - Kriwacki, Richard W.

PY - 2006/10

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AB - Intrinsically unstructured proteins (IUPs) represent an important class of proteins primarily involved in cellular signaling and regulation. The aim of this study was to develop methodology for the enrichment and identification of IUPs. We show that heat treatment of NIH3T3 mouse fibroblast cell extracts at 98 °C selects for IUPs. The majority of these IUPs were cytosolic or nuclear proteins involved in cell signaling or regulation. These studies represent the first large-scale experimental investigation of the intrinsically unstructured mammalian proteome.

KW - Disordered proteins

KW - Heat denaturation

KW - Intrinsically unstructured proteins

KW - Mammalian proteome

KW - Proteomics

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Proteomic studies of the intrinsically unstructured mammalian proteome

Abstract

Keywords

ASJC Scopus subject areas

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