Proteomic studies of the intrinsically unstructured mammalian proteome

Charles A. Galea, Vishwajeeth R. Pagala, John C. Obenauer, Cheon Gil Park, Clive A. Slaughter, Richard W. Kriwacki

Research output: Contribution to journalArticlepeer-review

58 Scopus citations


Intrinsically unstructured proteins (IUPs) represent an important class of proteins primarily involved in cellular signaling and regulation. The aim of this study was to develop methodology for the enrichment and identification of IUPs. We show that heat treatment of NIH3T3 mouse fibroblast cell extracts at 98 °C selects for IUPs. The majority of these IUPs were cytosolic or nuclear proteins involved in cell signaling or regulation. These studies represent the first large-scale experimental investigation of the intrinsically unstructured mammalian proteome.

Original languageEnglish (US)
Pages (from-to)2839-2848
Number of pages10
JournalJournal of Proteome Research
Issue number10
StatePublished - Oct 2006
Externally publishedYes


  • Disordered proteins
  • Heat denaturation
  • Intrinsically unstructured proteins
  • Mammalian proteome
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)


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