PYRIMIDINE DIMER FORMATION IN HUMAN SKIN

Steven E. Freeman; Richard W. Gange; John C. Sutherland; Betsy M. Sutherland

doi:10.1111/j.1751-1097.1987.tb04758.x

PYRIMIDINE DIMER FORMATION IN HUMAN SKIN

Steven E. Freeman, Richard W. Gange, John C. Sutherland, Betsy M. Sutherland

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Cyclobutyl pyrimidine dimers are major photoproducts formed upon irradiation of DNA with ultraviolet light. We have developed a method for detecting as few as one pyrimidine dimer per million bases in about 50 ng of non‐radioactive DNA, and have used this method to quantitate dimer yields in human skin DNA exposed in situ to UV. We found that UVA radiation (320–400 nm) produces detectable levels of dimers in the DNA of human skin. We also measured UVB‐induced dimer yields in skin of individuals of differing sun sensitivity and found higher yields in individuals with higher UVB minimal erythema doses and greater sun sensitivity. These approaches should provide important information on damage induced in human skin upon exposure to natural or artificial sources of ultraviolet radiation.

Original language	English (US)
Pages (from-to)	207-212
Number of pages	6
Journal	Photochemistry and Photobiology
Volume	46
Issue number	2
DOIs	https://doi.org/10.1111/j.1751-1097.1987.tb04758.x
State	Published - Aug 1987
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry

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title = "PYRIMIDINE DIMER FORMATION IN HUMAN SKIN",

abstract = "Cyclobutyl pyrimidine dimers are major photoproducts formed upon irradiation of DNA with ultraviolet light. We have developed a method for detecting as few as one pyrimidine dimer per million bases in about 50 ng of non‐radioactive DNA, and have used this method to quantitate dimer yields in human skin DNA exposed in situ to UV. We found that UVA radiation (320–400 nm) produces detectable levels of dimers in the DNA of human skin. We also measured UVB‐induced dimer yields in skin of individuals of differing sun sensitivity and found higher yields in individuals with higher UVB minimal erythema doses and greater sun sensitivity. These approaches should provide important information on damage induced in human skin upon exposure to natural or artificial sources of ultraviolet radiation.",

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AU - Freeman, Steven E.

AU - Gange, Richard W.

AU - Sutherland, John C.

AU - Sutherland, Betsy M.

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AB - Cyclobutyl pyrimidine dimers are major photoproducts formed upon irradiation of DNA with ultraviolet light. We have developed a method for detecting as few as one pyrimidine dimer per million bases in about 50 ng of non‐radioactive DNA, and have used this method to quantitate dimer yields in human skin DNA exposed in situ to UV. We found that UVA radiation (320–400 nm) produces detectable levels of dimers in the DNA of human skin. We also measured UVB‐induced dimer yields in skin of individuals of differing sun sensitivity and found higher yields in individuals with higher UVB minimal erythema doses and greater sun sensitivity. These approaches should provide important information on damage induced in human skin upon exposure to natural or artificial sources of ultraviolet radiation.

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PYRIMIDINE DIMER FORMATION IN HUMAN SKIN

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