TY - JOUR
T1 - QUANTITATION OF DNA DAMAGE IN NON‐RADIOACTIVE DNA
AU - Sutherland, Betsy M.
AU - Ciarrocchi, Giovanni
AU - Ciomei, Marina
AU - Sutherland, John C.
PY - 1986/9
Y1 - 1986/9
N2 - Three principal methods have been developed for measuring femtomoles of damage in nanogram quantities of non‐radioactive DNA. Lesions which can be quantified include single and double strand breaks, alkali labile sites including apurinic and apyrimidinic sites, and pyrimidine dimers. The first in vitro method measures the conversion of supercoiled DNA to relaxed or linear molecules, and can detect up to four lesions per molecule. The second in vitro method (supercoil depletion) assesses the fraction of intact linear molecules of homogeneous length, and allows detection of 8 lesions/molecule. The third method, measurement of molecular length distributions of DNAs of heterogeneous length, reveals the extent of DNA damage and repair in vivo or in vitro.
AB - Three principal methods have been developed for measuring femtomoles of damage in nanogram quantities of non‐radioactive DNA. Lesions which can be quantified include single and double strand breaks, alkali labile sites including apurinic and apyrimidinic sites, and pyrimidine dimers. The first in vitro method measures the conversion of supercoiled DNA to relaxed or linear molecules, and can detect up to four lesions per molecule. The second in vitro method (supercoil depletion) assesses the fraction of intact linear molecules of homogeneous length, and allows detection of 8 lesions/molecule. The third method, measurement of molecular length distributions of DNAs of heterogeneous length, reveals the extent of DNA damage and repair in vivo or in vitro.
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U2 - 10.1111/j.1751-1097.1986.tb04681.x
DO - 10.1111/j.1751-1097.1986.tb04681.x
M3 - Article
C2 - 3538069
AN - SCOPUS:0022772777
SN - 0031-8655
VL - 44
SP - 391
EP - 396
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
IS - 3
ER -