Quantitative IgE antibody assays in allergic diseases

John W. Yunginger, Staffan Ahlstedt, Peyton A. Eggleston, Henry A. Homburger, Harold S. Nelson, Dennis R. Ownby, Thomas A.E. Platts-Mills, Hugh A. Sampson, Scott H. Sicherer, Allan M. Weinstein, P. Brock Williams, Robert A. Wood, Robert S. Zeiger

Research output: Contribution to journalArticlepeer-review

170 Scopus citations


During the past several years, immunoassays for specific IgE antibodies have been refined to permit reporting results in mass units. Thus quantitative immunoassays for IgE antibodies may be an adjunct to skin tests. In cases of food allergy among children with atopic dermatitis, cutoff values for IgE antibody concentrations to egg, milk, peanut, and fish have been derived to provide 95% positive and 90% negative predictive values. Food-specific IgE antibody determinations can also be used to predict which food allergies are resolving spontaneously. Elevated egg-specific IgE antibody levels in infancy are associated with significantly increased risk for development of inhalant allergies later in childhood. In cases of inhalant allergy, specific IgE antibody levels correlate closely with results of inhalation challenge studies in cat-sensitive persons. Also, mite-specific IgE antibody levels correlate significantly with the mite allergen contents of reservoir dust in the homes of mite-sensitive persons. Immunoassays for quantitation of specific IgE antibodies may be used to document aller-gen sensitization over time and to evaluate the risk of reaction on allergen exposure. However, immunoassays and skin tests are not entirely interchangeable, and neither will replace the other in appropriate circumstances.

Original languageEnglish (US)
Pages (from-to)1077-1084
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Issue number6 II
StatePublished - 2000


  • Allergic disease
  • IgE antibody
  • Quantitative immunoassay

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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