TY - JOUR
T1 - Quantities of adult, fetal and embryonic globin chains in the blood of eighteen- to twenty-week-old human fetuses
AU - Kutlar, Ferdane
AU - Moscoso, Hugo
AU - Kiefer, Charles R.
AU - Garver, Frederick A.
AU - Beksaç, Sinan
AU - Önderoglu, Lütfi
AU - Gurgey, Aytemiz
AU - Altay, Çigdem
AU - Huisman, Titus H.J.
N1 - Funding Information:
This study was supportedi n part by USPHS ResearchG rant HLB-05168 (to T.H.J.H.) and by funds from the Prenatal Diagnosis Unit of the Turkish Scientific Research Foundation in Ankara, Turkey (to C.A. and A.G.). This is contribution No. 1246f rom the Departmento f Cell and Molecular Biology at the Medical College of Georgia in Augusta, GA, U.S.A.
PY - 1991/7/5
Y1 - 1991/7/5
N2 - The prenatal diagnostic program, established at Hacettepe University in Ankara for the purpose of detecting β-thalassemia (β-thal), sickle cell anemia (SS), and Hb S-β-thal, offered the opportunity of evaluating the relative quantities of adult (βA, βS), fetal (Gγ, Aγ, AγT), and embryonic (ε, ζ) chains in 26 fetuses, aged 18-20 weeks. Methodology involved micro high-performance liquid chromatographic (HPLC) procedures and immunology using an mAb, specific for the embryonic ε chain. A good correlation was observed between the β γ in vitro chain synthesis ratio and the level of βA and/or βS chains determined by reversed-phase HPLC; the combination of these two sets of data strengthens the prenatal diagnostic approach of detecting β-thal major but not β-thal trait. The levels of the different γ chains were about as observed in newborn babies; the frequency of the AγT variant in the 26 fetuses was the same as observed for a larger group of Turkisch newborn babies. The level of the embryonic ζ chain was higher than seen in full-term babies and varied between 0 and 1.3%; 5 of the 26 fetuses showed the complete absence of ζ. The embryonic ε chain was not detectable, not even in babies with β-thal major. These data indicate that the synthesis of ε is completely turned off in fetuses at the age of 18-20 weeks, while that of ζ continues, albeit at a low level.
AB - The prenatal diagnostic program, established at Hacettepe University in Ankara for the purpose of detecting β-thalassemia (β-thal), sickle cell anemia (SS), and Hb S-β-thal, offered the opportunity of evaluating the relative quantities of adult (βA, βS), fetal (Gγ, Aγ, AγT), and embryonic (ε, ζ) chains in 26 fetuses, aged 18-20 weeks. Methodology involved micro high-performance liquid chromatographic (HPLC) procedures and immunology using an mAb, specific for the embryonic ε chain. A good correlation was observed between the β γ in vitro chain synthesis ratio and the level of βA and/or βS chains determined by reversed-phase HPLC; the combination of these two sets of data strengthens the prenatal diagnostic approach of detecting β-thal major but not β-thal trait. The levels of the different γ chains were about as observed in newborn babies; the frequency of the AγT variant in the 26 fetuses was the same as observed for a larger group of Turkisch newborn babies. The level of the embryonic ζ chain was higher than seen in full-term babies and varied between 0 and 1.3%; 5 of the 26 fetuses showed the complete absence of ζ. The embryonic ε chain was not detectable, not even in babies with β-thal major. These data indicate that the synthesis of ε is completely turned off in fetuses at the age of 18-20 weeks, while that of ζ continues, albeit at a low level.
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U2 - 10.1016/0378-4347(91)80142-Y
DO - 10.1016/0378-4347(91)80142-Y
M3 - Article
C2 - 1939469
AN - SCOPUS:0025727938
SN - 0378-4347
VL - 567
SP - 359
EP - 368
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 2
ER -