TY - JOUR
T1 - Recurrent bacteremia caused by a 'Flexispira'-like organism in a patient with X-linked (Bruton's) agammaglobulinemia
AU - Weir, Susan
AU - Cuccherini, Brenda
AU - Whitney, Anne M.
AU - Ray, Marsha L.
AU - MacGregor, John P.
AU - Steigerwalt, Arnold
AU - Daneshvar, Maryam I.
AU - Weyant, Robbin
AU - Wray, Betty
AU - Steele, John
AU - Strober, Warren
AU - Gill, Vee J.
PY - 1999
Y1 - 1999
N2 - Helicobacter spp., except for Helicobacter cinaedi, have only rarely been reported in cases of septicemia. A patient with X-linked (Bruton's) agammaglobulinemia was found to have persistent sepsis with a Helicobacter- like organism despite multiple courses of antibiotics. His periods of sepsis were associated with leg swelling thought to be consistent with cellulitis. The organism was fastidious and required a microaerophilic environment containing H2 for growth. Optimal growth was observed at 35 to 37°C on sheep blood, CDC anaerobe, and Bordet-Gengou agars. Serial subcultures every 4 to 5 days were required to maintain viability. The organism was strongly urease positive and showed highest relatedness to Helicobacter-like organisms with the vernacular name 'Flexispira rappini' by 16S rRNA gene sequence analysis. Genomic DNA hybridization studies, however, found 24 to 37% relatedness to 'F. rappini' and even less to other Helicobacter spp. Although the organism phenotypically resembles 'Flexispira' and Helicobacter, it is thought to represent a new taxon. The patient's infection was eventually cleared with a prolonged (5-month) course of intravenous imipenem and gentamicin.
AB - Helicobacter spp., except for Helicobacter cinaedi, have only rarely been reported in cases of septicemia. A patient with X-linked (Bruton's) agammaglobulinemia was found to have persistent sepsis with a Helicobacter- like organism despite multiple courses of antibiotics. His periods of sepsis were associated with leg swelling thought to be consistent with cellulitis. The organism was fastidious and required a microaerophilic environment containing H2 for growth. Optimal growth was observed at 35 to 37°C on sheep blood, CDC anaerobe, and Bordet-Gengou agars. Serial subcultures every 4 to 5 days were required to maintain viability. The organism was strongly urease positive and showed highest relatedness to Helicobacter-like organisms with the vernacular name 'Flexispira rappini' by 16S rRNA gene sequence analysis. Genomic DNA hybridization studies, however, found 24 to 37% relatedness to 'F. rappini' and even less to other Helicobacter spp. Although the organism phenotypically resembles 'Flexispira' and Helicobacter, it is thought to represent a new taxon. The patient's infection was eventually cleared with a prolonged (5-month) course of intravenous imipenem and gentamicin.
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U2 - 10.1128/jcm.37.8.2439-2445.1999
DO - 10.1128/jcm.37.8.2439-2445.1999
M3 - Article
C2 - 10405381
AN - SCOPUS:0032773377
SN - 0095-1137
VL - 37
SP - 2439
EP - 2445
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 8
ER -