Abstract
CpG motifs have been advanced as agents that stimulate the maturation of DCs for immunotherapy. The present study tested the hypothesis that multiple doses of CpG-matured DC vaccine would be efficacious for complete eradication of experimentally-induced tumor. Accordingly, WEHI164 cells were implanted subcutaneously in the flank of BALB/c mice. During DC culture, tumor lysate was added to immature DCs followed by addition of CpG or non-CpG control 4-6 h later. A total of three doses of CpG or non-CpG control-matured DCs were injected around tumors. The results showed that multiple doses of CpG-matured DCs led to considerable decrease in cytotoxicity of lymphocytes and significantly increased tumor growth rate compared to a single dose. Further, mice which received three doses of the vaccine also displayed significant FoxP3 in tumor tissue. In conclusion, multiple doses of CpG-matured DCs exhibited decreased anti-tumor immunity in association with increased expression of FoxP3.
Original language | English (US) |
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Pages (from-to) | 360-364 |
Number of pages | 5 |
Journal | Cellular Immunology |
Volume | 271 |
Issue number | 2 |
DOIs | |
State | Published - 2011 |
Keywords
- Cell therapy
- FoxP3
- Synthetic oligodeoxynucleotides
- Tumor immunotherapy
ASJC Scopus subject areas
- Immunology