Reduction in Antimicrobial Substantivity of MTAD After Initial Sodium Hypochlorite Irrigation

Franklin R. Tay; Noriko Hiraishi; George S. Schuster; David H. Pashley; Robert J. Loushine; Hani F. Ounsi; Simone Grandini; Joyce Y.Y. Yau; Annalisa Mazzoni; Adam Donnelly; Nigel M. King

doi:10.1016/j.joen.2006.03.016

Reduction in Antimicrobial Substantivity of MTAD After Initial Sodium Hypochlorite Irrigation

Franklin R. Tay, Noriko Hiraishi, George S. Schuster, David H. Pashley, Robert J. Loushine, Hani F. Ounsi, Simone Grandini, Joyce Y.Y. Yau, Annalisa Mazzoni, Adam Donnelly, Nigel M. King

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32 Scopus citations

Abstract

Potential intrinsic tetracycline staining of intraradicular dentin has been observed when BioPure MTAD was employed as the final irrigant after initial rinsing with NaOCl. This study examined the effect of NaOCl-MTAD interaction on the antimicrobial substantivity of MTAD in dentin. Dentin cores previously irrigated with either MTAD, or in conjunction with 1.3% NaOCl as an initial irrigant were placed on blood agar plates inoculated with Escherichia faecalis at 10⁵ cfu/ml. Dentin cores irrigated with 1.3% NaOCl only, and autoclaved dentin disks were used as the respective positive and negative controls. After anaerobic incubation, the mean diameter of bacterial inhibition zones formed around the MTAD group was significantly larger than the NaOCl/MTAD group, which, in turn, was not significantly different from the NaOCl positive control. Oxidation of MTAD by NaOCl resulted in the partial loss of antimicrobial substantivity in a manner similar to the peroxidation of tetracycline by reactive oxygen species.

Original language	English (US)
Pages (from-to)	970-975
Number of pages	6
Journal	Journal of endodontics
Volume	32
Issue number	10
DOIs	https://doi.org/10.1016/j.joen.2006.03.016
State	Published - Oct 2006

Keywords

Antimicrobial potency
Escherichia faecalis
MTAD
doxycycline
oxidation
sodium hypochlorite

ASJC Scopus subject areas

General Dentistry

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10.1016/j.joen.2006.03.016

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@article{aa1f0801379f4a7a8cec5b740aff5b5b,

title = "Reduction in Antimicrobial Substantivity of MTAD After Initial Sodium Hypochlorite Irrigation",

abstract = "Potential intrinsic tetracycline staining of intraradicular dentin has been observed when BioPure MTAD was employed as the final irrigant after initial rinsing with NaOCl. This study examined the effect of NaOCl-MTAD interaction on the antimicrobial substantivity of MTAD in dentin. Dentin cores previously irrigated with either MTAD, or in conjunction with 1.3% NaOCl as an initial irrigant were placed on blood agar plates inoculated with Escherichia faecalis at 105 cfu/ml. Dentin cores irrigated with 1.3% NaOCl only, and autoclaved dentin disks were used as the respective positive and negative controls. After anaerobic incubation, the mean diameter of bacterial inhibition zones formed around the MTAD group was significantly larger than the NaOCl/MTAD group, which, in turn, was not significantly different from the NaOCl positive control. Oxidation of MTAD by NaOCl resulted in the partial loss of antimicrobial substantivity in a manner similar to the peroxidation of tetracycline by reactive oxygen species.",

keywords = "Antimicrobial potency, Escherichia faecalis, MTAD, doxycycline, oxidation, sodium hypochlorite",

author = "Tay, {Franklin R.} and Noriko Hiraishi and Schuster, {George S.} and Pashley, {David H.} and Loushine, {Robert J.} and Ounsi, {Hani F.} and Simone Grandini and Yau, {Joyce Y.Y.} and Annalisa Mazzoni and Adam Donnelly and King, {Nigel M.}",

note = "Funding Information: This study was supported by R01 grants DE 014911 and DE 015306 from NIDCR, USA (PI David Pashley) and by grant 2004356/90800/08004/400/01, Faculty of Dentistry, University of Hong Kong. The authors graciously acknowledge the technical assistance of Dr. Yoshihiro Nishitani and the secretarial support provided by Michelle Barnes, Zinnia Ng, Debbie Dye, and Jane Flanders.",

year = "2006",

month = oct,

doi = "10.1016/j.joen.2006.03.016",

language = "English (US)",

volume = "32",

pages = "970--975",

journal = "Journal of endodontics",

issn = "0099-2399",

publisher = "Elsevier Inc.",

number = "10",

}

TY - JOUR

T1 - Reduction in Antimicrobial Substantivity of MTAD After Initial Sodium Hypochlorite Irrigation

AU - Tay, Franklin R.

AU - Hiraishi, Noriko

AU - Schuster, George S.

AU - Pashley, David H.

AU - Loushine, Robert J.

AU - Ounsi, Hani F.

AU - Grandini, Simone

AU - Yau, Joyce Y.Y.

AU - Mazzoni, Annalisa

AU - Donnelly, Adam

AU - King, Nigel M.

N1 - Funding Information: This study was supported by R01 grants DE 014911 and DE 015306 from NIDCR, USA (PI David Pashley) and by grant 2004356/90800/08004/400/01, Faculty of Dentistry, University of Hong Kong. The authors graciously acknowledge the technical assistance of Dr. Yoshihiro Nishitani and the secretarial support provided by Michelle Barnes, Zinnia Ng, Debbie Dye, and Jane Flanders.

PY - 2006/10

Y1 - 2006/10

N2 - Potential intrinsic tetracycline staining of intraradicular dentin has been observed when BioPure MTAD was employed as the final irrigant after initial rinsing with NaOCl. This study examined the effect of NaOCl-MTAD interaction on the antimicrobial substantivity of MTAD in dentin. Dentin cores previously irrigated with either MTAD, or in conjunction with 1.3% NaOCl as an initial irrigant were placed on blood agar plates inoculated with Escherichia faecalis at 105 cfu/ml. Dentin cores irrigated with 1.3% NaOCl only, and autoclaved dentin disks were used as the respective positive and negative controls. After anaerobic incubation, the mean diameter of bacterial inhibition zones formed around the MTAD group was significantly larger than the NaOCl/MTAD group, which, in turn, was not significantly different from the NaOCl positive control. Oxidation of MTAD by NaOCl resulted in the partial loss of antimicrobial substantivity in a manner similar to the peroxidation of tetracycline by reactive oxygen species.

AB - Potential intrinsic tetracycline staining of intraradicular dentin has been observed when BioPure MTAD was employed as the final irrigant after initial rinsing with NaOCl. This study examined the effect of NaOCl-MTAD interaction on the antimicrobial substantivity of MTAD in dentin. Dentin cores previously irrigated with either MTAD, or in conjunction with 1.3% NaOCl as an initial irrigant were placed on blood agar plates inoculated with Escherichia faecalis at 105 cfu/ml. Dentin cores irrigated with 1.3% NaOCl only, and autoclaved dentin disks were used as the respective positive and negative controls. After anaerobic incubation, the mean diameter of bacterial inhibition zones formed around the MTAD group was significantly larger than the NaOCl/MTAD group, which, in turn, was not significantly different from the NaOCl positive control. Oxidation of MTAD by NaOCl resulted in the partial loss of antimicrobial substantivity in a manner similar to the peroxidation of tetracycline by reactive oxygen species.

KW - Antimicrobial potency

KW - Escherichia faecalis

KW - MTAD

KW - doxycycline

KW - oxidation

KW - sodium hypochlorite

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AN - SCOPUS:33748536451

SN - 0099-2399

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SP - 970

EP - 975

JO - Journal of endodontics

JF - Journal of endodontics

IS - 10

ER -

Reduction in Antimicrobial Substantivity of MTAD After Initial Sodium Hypochlorite Irrigation

Abstract

Keywords

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