TY - JOUR
T1 - Regulation of equine herpesvirus type 1 gene expression
T2 - Characterization of immediate early, early, and late transcription
AU - Gray, Wayne L.
AU - Baumann, Raymond P.
AU - Robertson, Alice T.
AU - Caughman, Gretchen B.
AU - O'Callaghan, Dennis J.
AU - Staczek, John
N1 - Funding Information:
We thank Angela Houston and Cynthia Harrison for typing the manuscript, and Mr. Chandra Sharma for technical assistance. Support for this investigation was obtained from Research Grants Al21996, Al22001, Al22894, 1 F32 CA 07700-01, and 2S07RR05822-07 from the National Institutes of Health, a Grayson Foundation Inc. research grant, and a grant from the L.S.U. Biotechnology Institute.
PY - 1987/5
Y1 - 1987/5
N2 - The regulation of equine herpesvirus type 1 (EHV-1) transcription was examined in infected rabbit kidney cells using metabolic inhibitors. In order to map EHV-1 immediate early, early, and late transcripts, viral RNA was 32P-labeled in vivo and hybridized to EHV-1 DNA restriction fragments immobilized on nitrocellulose filters. Immediate early viral RNA was mapped to one region of the viral genome within the inverted repeat DNA sequences (map units 0.78-0.83 and 0.95-1.0). Northern blot hybridization analysis using a 32P-labeled cloned DNA probe from this region identified a single immediate early viral transcript (approximately 6 kb). Transcription of early and late genes was not restricted to any specific region on the viral genome as indicated by the ability of 32P-labeled early and late RNA to hybridize to EHV-1 restriction endonuclease fragments from both the long and short components of EHV-1 DNA. Additional experiments performed without the use of metabolic inhibitors confirmed that EHV-1 transcription is temporally regulated. The characterization of EHV-1 transcription during productive infection will serve as a reference for the analysis of viral transcripts in oncogenically transformed and persistently infected cells.
AB - The regulation of equine herpesvirus type 1 (EHV-1) transcription was examined in infected rabbit kidney cells using metabolic inhibitors. In order to map EHV-1 immediate early, early, and late transcripts, viral RNA was 32P-labeled in vivo and hybridized to EHV-1 DNA restriction fragments immobilized on nitrocellulose filters. Immediate early viral RNA was mapped to one region of the viral genome within the inverted repeat DNA sequences (map units 0.78-0.83 and 0.95-1.0). Northern blot hybridization analysis using a 32P-labeled cloned DNA probe from this region identified a single immediate early viral transcript (approximately 6 kb). Transcription of early and late genes was not restricted to any specific region on the viral genome as indicated by the ability of 32P-labeled early and late RNA to hybridize to EHV-1 restriction endonuclease fragments from both the long and short components of EHV-1 DNA. Additional experiments performed without the use of metabolic inhibitors confirmed that EHV-1 transcription is temporally regulated. The characterization of EHV-1 transcription during productive infection will serve as a reference for the analysis of viral transcripts in oncogenically transformed and persistently infected cells.
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U2 - 10.1016/0042-6822(87)90240-6
DO - 10.1016/0042-6822(87)90240-6
M3 - Article
C2 - 3033896
AN - SCOPUS:0023277358
SN - 0042-6822
VL - 158
SP - 79
EP - 87
JO - Virology
JF - Virology
IS - 1
ER -