TY - JOUR
T1 - Resin adhesion to carious dentin
AU - Yoshiyama, Masahiro
AU - Tay, Franklin R.
AU - Torii, Yasuhiro
AU - Nishitani, Yoshihiro
AU - Doi, Junichi
AU - Itou, Kousuke
AU - Ciucchi, Bernard
AU - Pashley, David H.
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2003/2
Y1 - 2003/2
N2 - Purpose: To investigate the microtensile bond strength (μTBS) of a self-etching priming adhesive system to normal, caries-affected and caries-infected dentin, and to observe the ultrastructure of the resin-dentin interface by transmission electron microscopy (TEM). Materials and Methods: Twelve extracted human molar teeth with deep occlusal caries were stained with caries detector solution and ground flat occlusally. The red-stained soft dentin was classified as caries-infected. The surrounding discolored dentin was classified as caries-affected dentin. The surrounding normal dentin served as a control. The entire flat surface was bonded with Clearfil Liner Bond 2V (CV) and covered with resin composite to form a composite crown 5 mm high. One day later the specimens were serially sectioned vertically into multiple slabs 0.8 mm thick. Under microscopic observation, the specimens were divided into normal or caries-infected or caries-affected dentin. These regions were isolated by cutting away the remaining dentin to form hour-glass shapes with the smallest surface area at the test site. After measuring the areas, the specimens were fixed to a microtensile tester and pulled under tension to failure. Additional slabs that were not used for bond strength tests were processed for TEM. Bond strength data were analyzed by Kruskal-Wallis one-way ANOVA and Student-Newman-Keuls multiple comparisons. Results: The μTBS of CV to normal, caries-affected and caries-infected dentin were 45 ± 10 MPa, 30 ± 10 MPa, 10 ± 5 MPa, respectively. TEM images showed that CV formed thin hybrid layers that were less than 1 μm thick in normal dentin, but that were between 6-8 μm thick in caries-affected dentin. Bacteria were only sparsely observed in the dentin tubules of bonded caries-affected dentin. However, in caries-infected dentin, an unusual interface was seen in which carious bacteria within disorganized non-banded collagen fibrils could be seen embedded by the adhesive. The hybrid layer in caries-infected dentin was found to be 30-60 μm thick.
AB - Purpose: To investigate the microtensile bond strength (μTBS) of a self-etching priming adhesive system to normal, caries-affected and caries-infected dentin, and to observe the ultrastructure of the resin-dentin interface by transmission electron microscopy (TEM). Materials and Methods: Twelve extracted human molar teeth with deep occlusal caries were stained with caries detector solution and ground flat occlusally. The red-stained soft dentin was classified as caries-infected. The surrounding discolored dentin was classified as caries-affected dentin. The surrounding normal dentin served as a control. The entire flat surface was bonded with Clearfil Liner Bond 2V (CV) and covered with resin composite to form a composite crown 5 mm high. One day later the specimens were serially sectioned vertically into multiple slabs 0.8 mm thick. Under microscopic observation, the specimens were divided into normal or caries-infected or caries-affected dentin. These regions were isolated by cutting away the remaining dentin to form hour-glass shapes with the smallest surface area at the test site. After measuring the areas, the specimens were fixed to a microtensile tester and pulled under tension to failure. Additional slabs that were not used for bond strength tests were processed for TEM. Bond strength data were analyzed by Kruskal-Wallis one-way ANOVA and Student-Newman-Keuls multiple comparisons. Results: The μTBS of CV to normal, caries-affected and caries-infected dentin were 45 ± 10 MPa, 30 ± 10 MPa, 10 ± 5 MPa, respectively. TEM images showed that CV formed thin hybrid layers that were less than 1 μm thick in normal dentin, but that were between 6-8 μm thick in caries-affected dentin. Bacteria were only sparsely observed in the dentin tubules of bonded caries-affected dentin. However, in caries-infected dentin, an unusual interface was seen in which carious bacteria within disorganized non-banded collagen fibrils could be seen embedded by the adhesive. The hybrid layer in caries-infected dentin was found to be 30-60 μm thick.
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M3 - Article
C2 - 12744413
AN - SCOPUS:0042020372
SN - 0894-8275
VL - 16
SP - 47
EP - 52
JO - American journal of dentistry
JF - American journal of dentistry
IS - 1
ER -