TY - JOUR
T1 - Role of arginase 2 in murine retinopathy associated with western diet‐induced obesity
AU - Atawia, Reem T.
AU - Bunch, Katharine L.
AU - Fouda, Abdelrahman Y.
AU - Lemtalsi, Tahira
AU - Eldahshan, Wael
AU - Xu, Zhimin
AU - Saul, Alan
AU - Elmasry, Khaled
AU - Al‐shabrawey, Mohamed
AU - Caldwell, Ruth B.
AU - Caldwell, R. William
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/2
Y1 - 2020/2
N2 - Western diet‐induced obesity is linked to the development of metabolic dysfunctions, including type 2 diabetes and complications that include retinopathy, a leading cause of blindness. Aberrant activation of the inflammasome cascade leads to the progression of obesity‐induced pathologies. Our lab showed the critical role of arginase 2 (A2), the mitochondrial isoform of this ureahydrolase, in obesity‐induced metabolic dysfunction and inflammation. A2 deletion also has been shown to be protective against retinal inflammation in models of ischemic retinopathy and multiple sclerosis. We investigated the effect of A2 deletion on western diet‐induced retinopathy. Wild‐type mice fed a high‐fat, high‐sucrose western diet for 16 weeks exhibited elevated retinal expression of A2, markers of the inflammasome pathway, oxidative stress, and activation of microglia/macrophages. Western diet feeding induced exaggerated retinal light responses without affecting visual acuity or retinal morphology. These effects were reduced or absent in mice with global A2 deletion. Exposure of retinal endothelial cells to palmitate and high glucose, a mimic of the obese state, increased expression of A2 and inflammatory mediators and induced cell death. These effects, except for A2, were prevented by pretreatment with an arginase inhibitor. Collectively, our study demonstrated a substantial role of A2 in early manifestations of diabetic retinopathy.
AB - Western diet‐induced obesity is linked to the development of metabolic dysfunctions, including type 2 diabetes and complications that include retinopathy, a leading cause of blindness. Aberrant activation of the inflammasome cascade leads to the progression of obesity‐induced pathologies. Our lab showed the critical role of arginase 2 (A2), the mitochondrial isoform of this ureahydrolase, in obesity‐induced metabolic dysfunction and inflammation. A2 deletion also has been shown to be protective against retinal inflammation in models of ischemic retinopathy and multiple sclerosis. We investigated the effect of A2 deletion on western diet‐induced retinopathy. Wild‐type mice fed a high‐fat, high‐sucrose western diet for 16 weeks exhibited elevated retinal expression of A2, markers of the inflammasome pathway, oxidative stress, and activation of microglia/macrophages. Western diet feeding induced exaggerated retinal light responses without affecting visual acuity or retinal morphology. These effects were reduced or absent in mice with global A2 deletion. Exposure of retinal endothelial cells to palmitate and high glucose, a mimic of the obese state, increased expression of A2 and inflammatory mediators and induced cell death. These effects, except for A2, were prevented by pretreatment with an arginase inhibitor. Collectively, our study demonstrated a substantial role of A2 in early manifestations of diabetic retinopathy.
KW - Arginase 2
KW - Inflammasome
KW - Obesity/diabetes‐induced retinopathy
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=85099399369&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85099399369&partnerID=8YFLogxK
U2 - 10.3390/jcm9020317
DO - 10.3390/jcm9020317
M3 - Article
AN - SCOPUS:85099399369
SN - 2077-0383
VL - 9
JO - Journal of Clinical Medicine
JF - Journal of Clinical Medicine
IS - 2
M1 - 317
ER -