Role of dentin cross-linking agents in optimizing dentin bond durability

M. Abunawareg, D. A. Abuelenain, D. Elkassas, T. Abu Haimed, A. Al-Dharrab, A. Zidan, A. H. Hassan, David Henry Pashley

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The present work compared the effects of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC-HCl) and ultraviolet (UV)- or blue light-activated riboflavin cross-linking agents on resin–dentin micro-tensile bond strength and nanoleakage in bonds produced using a two-step, etch-and-rinse adhesive after three storage intervals (24 h, 6 months, and 12 months). Forty eight extracted human third molars were used to investigate micro-tensile bond strength and interfacial nanoleakage in resin–dentin bonds created using Adper Single Bond 2, with or without pretreatment of acid-etched dentin surfaces. Either 0.5 M EDC-HCl or 1% riboflavin-5-phosphate activated by blue or UV light were used as cross-linking agents. Samples were evaluated after storage for 24 h, 6 months, or 12 months in distilled water at 37 °C. Statistical analyses revealed that 12 months of storage resulted in significant decreases in the bond strength of the controls (p < 0.003), with significant increases in their silver nanoleakage (p < 0.05), compared with the groups subjected to dentin pretreatment with either EDC-HCl or light-activated 1% riboflavin. Despite the significant drop in bond strength after 6 months’ storage in all experimental groups compared with the 24-h bond strength (p < 0.05), there was a further non-significant drop in bond strength after 12 months in samples treated with EDC-HCl and UV-activated 1% riboflavin (p > 0.05). Dental collagen cross-linking induced by UV- or blue light-activated 1% riboflavin or EDC-HCl enhanced the durability and strength of the resin–dentin bond.

Original languageEnglish (US)
Pages (from-to)83-88
Number of pages6
JournalInternational Journal of Adhesion and Adhesives
StatePublished - Oct 2017


  • Hybrid layer
  • Micro-tensile
  • Nanoleakage
  • Riboflavin
  • Surface treatment

ASJC Scopus subject areas

  • Biomaterials
  • General Chemical Engineering
  • Polymers and Plastics


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