Abstract
Objective: Study the expression pattern of tissue-type (t-PA) and urokinase-type (u-PA) plasminogen activators during uterine serosal healing after lesioning in a rat model. Design: Descriptive study with an adhesion model using reverse transcriptase-polymerase chain reaction (PCR) and immuncytochemical techniques. Setting: Academic medical center. Patient(s): None. Intervention(s): None. Main Outcome Measure(s): Expression profiles of t-PA and u-PA were examined in the uteroperitoneal adhesion tissues of rat from the time of injury until day 7 after surgery using relative abundance reverse transcriptase-PCR and immunocytochemical techniques. Result(s): The t-PA transcript levels dropped in uteroperitoneal tissues by 6 hours after surgery and remained low until day 5. Thereafter, a rapid up-regulation of this transcript was observed at day 7. In contrast, the u-PA transcript levels demonstrated a biphasic profile during the progression of adhesion development with peaks at 2 and 5 days after surgery. Immunocytochemical staining demonstrated expression of these plasminogen activators in the newly formed blood vessels. Conclusion(s): Altered levels of t-PA and u-PA transcripts and its expression in newly formed blood vessels during healing and adhesion development indicate involvement of these plasminogen activators in serosal healing and adhesion development.
Original language | English (US) |
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Pages (from-to) | 138-145 |
Number of pages | 8 |
Journal | Fertility and sterility |
Volume | 79 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 2003 |
Externally published | Yes |
Keywords
- Adhesion
- Angiogenesis
- Immunocytochemistry
- Rat
- Reverse transcriptase-PCR
- t-PA
- u-PA
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology