Abstract
To investigate the molecular basis of mononuclear cell rolling and adhesions to vascular endothelium prone to develop atherosclerotic lesion, isolated carotid arteries from ApoE-/- and other relevant mice were perfused under physiological shear stress conditions. Carotid arteries from 10-12 weeks old ApoE-/- and C57BL/6 wide-type mice fed a Western diet for 4-5 weeks, representing early atherosclerotic lesions according to vessel histology, supported mononuclear cell (U937) attachment, rolling and adhesion, while carotid arteries from an atherosclerosis-resistant strain (BALB/C) showed no adhesion. Antibody blocking assays showed that mononuclear rolling and adhesion were significantly inhibited by treating the vessel with P-selectin antibody or treating U937 with anti-PSGL-1. Treating the vessel with VCAM-1 antibody or treating U937 with α4β1 integrin antibody caused rolling velocities to increase (from 86±4 to 167±7 um/s). This suggests that mononuclear cell can attach, roll and adhere on early atherosclerotic endothelium via the P-selectin-PSGL-1 and VCAM-1-α4β1 pathways.
| Original language | English (US) |
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| Title of host publication | Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings |
| Publisher | IEEE |
| Number of pages | 1 |
| Volume | 1 |
| ISBN (Print) | 0780356756 |
| State | Published - Dec 1 1999 |
| Externally published | Yes |
| Event | Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA Duration: Oct 13 1999 → Oct 16 1999 |
Other
| Other | Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) |
|---|---|
| City | Atlanta, GA, USA |
| Period | 10/13/99 → 10/16/99 |
ASJC Scopus subject areas
- Bioengineering