Rutin protects the neural damage induced by transient focal ischemia in rats

Mohd Moshahid Khan; Ajmal Ahmad; Tauheed Ishrat; Gulrana Khuwaja; Pallavi Srivastawa; Mohammad Badruzzaman Khan; Syed Shadab Raza; Hayate Javed; Kumar Vaibhav; Andleeb Khan; Fakhrul Islam

doi:10.1016/j.brainres.2009.07.026

Rutin protects the neural damage induced by transient focal ischemia in rats

Mohd Moshahid Khan, Ajmal Ahmad, Tauheed Ishrat, Gulrana Khuwaja, Pallavi Srivastawa, Mohammad Badruzzaman Khan, Syed Shadab Raza, Hayate Javed, Kumar Vaibhav, Andleeb Khan, Fakhrul Islam

Research output: Contribution to journal › Article › peer-review

181 Scopus citations

Abstract

Free radical induced neural damage is implicated in cerebral ischemia-reperfusion (IR) injury and antioxidants are reported to have neuroprotective activity. The present study was designed to assess the neuroprotective role of rutin (Vitamin P), and mechanism of action. The middle cerebral artery (MCA) of an adult male Wistar rat was occluded for 2 h and reperfused for 22 h. The administration of rutin (25 mg/kg bwt., orally) once daily for 21 days before middle cerebral artery occlusion (MCAO) showed marked reduction in infarct size, reduced the neurological deficits in terms of behaviors, suppressed neuronal loss and diminished the p53 expression in MCAO rats. A significantly depleted activity of antioxidant enzymes, glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD) and content of glutathione (GSH) in MCAO group were protected significantly in MCAO group pretreated with rutin. Conversely, the elevated level of thiobarbituric acid reactive species (TBARS), H₂O₂ and protein carbonyl (PC) in MCAO group was attenuated significantly in rutin-pretreated group when compared with MCAO group. These results indicate that rutin attenuates ischemic neural apoptosis by reducing the expression of p53, preventing morphological changes and increasing endogenous antioxidant enzymatic activities. Thus, rutin treatment may represent a novel approach in lowering the risk or improving the function of ischemia-reperfusion brain injury-related disorders.

Original language	English (US)
Pages (from-to)	123-135
Number of pages	13
Journal	Brain Research
Volume	1292
DOIs	https://doi.org/10.1016/j.brainres.2009.07.026
State	Published - Sep 25 2009

Keywords

Apoptosis
Brain infarct
Ischemia
Neurobehavior
Oxidative stress
Rutin

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/j.brainres.2009.07.026

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@article{ea59d723a2da4172899c147e79b55596,

title = "Rutin protects the neural damage induced by transient focal ischemia in rats",

abstract = "Free radical induced neural damage is implicated in cerebral ischemia-reperfusion (IR) injury and antioxidants are reported to have neuroprotective activity. The present study was designed to assess the neuroprotective role of rutin (Vitamin P), and mechanism of action. The middle cerebral artery (MCA) of an adult male Wistar rat was occluded for 2 h and reperfused for 22 h. The administration of rutin (25 mg/kg bwt., orally) once daily for 21 days before middle cerebral artery occlusion (MCAO) showed marked reduction in infarct size, reduced the neurological deficits in terms of behaviors, suppressed neuronal loss and diminished the p53 expression in MCAO rats. A significantly depleted activity of antioxidant enzymes, glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD) and content of glutathione (GSH) in MCAO group were protected significantly in MCAO group pretreated with rutin. Conversely, the elevated level of thiobarbituric acid reactive species (TBARS), H2O2 and protein carbonyl (PC) in MCAO group was attenuated significantly in rutin-pretreated group when compared with MCAO group. These results indicate that rutin attenuates ischemic neural apoptosis by reducing the expression of p53, preventing morphological changes and increasing endogenous antioxidant enzymatic activities. Thus, rutin treatment may represent a novel approach in lowering the risk or improving the function of ischemia-reperfusion brain injury-related disorders.",

keywords = "Apoptosis, Brain infarct, Ischemia, Neurobehavior, Oxidative stress, Rutin",

author = "Khan, {Mohd Moshahid} and Ajmal Ahmad and Tauheed Ishrat and Gulrana Khuwaja and Pallavi Srivastawa and Khan, {Mohammad Badruzzaman} and Raza, {Syed Shadab} and Hayate Javed and Kumar Vaibhav and Andleeb Khan and Fakhrul Islam",

note = "Funding Information: The authors thank the Department of Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homoeopathy (AYUSH), Ministry of Health and Family Welfare, Government of India, New Delhi, for the financial assistance. Technical assistance of Dharamvir Singh is greatly acknowledged.",

year = "2009",

month = sep,

day = "25",

doi = "10.1016/j.brainres.2009.07.026",

language = "English (US)",

volume = "1292",

pages = "123--135",

journal = "Brain Research",

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TY - JOUR

T1 - Rutin protects the neural damage induced by transient focal ischemia in rats

AU - Khan, Mohd Moshahid

AU - Ahmad, Ajmal

AU - Ishrat, Tauheed

AU - Khuwaja, Gulrana

AU - Srivastawa, Pallavi

AU - Khan, Mohammad Badruzzaman

AU - Raza, Syed Shadab

AU - Javed, Hayate

AU - Vaibhav, Kumar

AU - Khan, Andleeb

AU - Islam, Fakhrul

N1 - Funding Information: The authors thank the Department of Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homoeopathy (AYUSH), Ministry of Health and Family Welfare, Government of India, New Delhi, for the financial assistance. Technical assistance of Dharamvir Singh is greatly acknowledged.

PY - 2009/9/25

Y1 - 2009/9/25

N2 - Free radical induced neural damage is implicated in cerebral ischemia-reperfusion (IR) injury and antioxidants are reported to have neuroprotective activity. The present study was designed to assess the neuroprotective role of rutin (Vitamin P), and mechanism of action. The middle cerebral artery (MCA) of an adult male Wistar rat was occluded for 2 h and reperfused for 22 h. The administration of rutin (25 mg/kg bwt., orally) once daily for 21 days before middle cerebral artery occlusion (MCAO) showed marked reduction in infarct size, reduced the neurological deficits in terms of behaviors, suppressed neuronal loss and diminished the p53 expression in MCAO rats. A significantly depleted activity of antioxidant enzymes, glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD) and content of glutathione (GSH) in MCAO group were protected significantly in MCAO group pretreated with rutin. Conversely, the elevated level of thiobarbituric acid reactive species (TBARS), H2O2 and protein carbonyl (PC) in MCAO group was attenuated significantly in rutin-pretreated group when compared with MCAO group. These results indicate that rutin attenuates ischemic neural apoptosis by reducing the expression of p53, preventing morphological changes and increasing endogenous antioxidant enzymatic activities. Thus, rutin treatment may represent a novel approach in lowering the risk or improving the function of ischemia-reperfusion brain injury-related disorders.

AB - Free radical induced neural damage is implicated in cerebral ischemia-reperfusion (IR) injury and antioxidants are reported to have neuroprotective activity. The present study was designed to assess the neuroprotective role of rutin (Vitamin P), and mechanism of action. The middle cerebral artery (MCA) of an adult male Wistar rat was occluded for 2 h and reperfused for 22 h. The administration of rutin (25 mg/kg bwt., orally) once daily for 21 days before middle cerebral artery occlusion (MCAO) showed marked reduction in infarct size, reduced the neurological deficits in terms of behaviors, suppressed neuronal loss and diminished the p53 expression in MCAO rats. A significantly depleted activity of antioxidant enzymes, glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD) and content of glutathione (GSH) in MCAO group were protected significantly in MCAO group pretreated with rutin. Conversely, the elevated level of thiobarbituric acid reactive species (TBARS), H2O2 and protein carbonyl (PC) in MCAO group was attenuated significantly in rutin-pretreated group when compared with MCAO group. These results indicate that rutin attenuates ischemic neural apoptosis by reducing the expression of p53, preventing morphological changes and increasing endogenous antioxidant enzymatic activities. Thus, rutin treatment may represent a novel approach in lowering the risk or improving the function of ischemia-reperfusion brain injury-related disorders.

KW - Apoptosis

KW - Brain infarct

KW - Ischemia

KW - Neurobehavior

KW - Oxidative stress

KW - Rutin

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U2 - 10.1016/j.brainres.2009.07.026

DO - 10.1016/j.brainres.2009.07.026

M3 - Article

C2 - 19631195

AN - SCOPUS:69449089391

SN - 0006-8993

VL - 1292

SP - 123

EP - 135

JO - Brain Research

JF - Brain Research

ER -

Rutin protects the neural damage induced by transient focal ischemia in rats

Abstract

Keywords

ASJC Scopus subject areas

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