TY - JOUR
T1 - Salvianolic Acids for Injection (SAFI) suppresses inflammatory responses in activated microglia to attenuate brain damage in focal cerebral ischemia
AU - Zhuang, Pengwei
AU - Wan, Yanjun
AU - Geng, Shihan
AU - He, Ying
AU - Ju, Aichun
N1 - Funding Information:
This work was supported by the National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program (2012ZX09101202), Program for innovation team training in universities in Tianjin (TD12-5035), The National Natural Science Foundation of China (81403213, 81673707), and Changjiang Scholars and Innovative Research Team in University (“PCSIRT” IRT 14R41).
Publisher Copyright:
© 2017 Elsevier Ireland Ltd
PY - 2017/2/23
Y1 - 2017/2/23
N2 - Background Inflammatory reactions induced by microglia in the brain play crucial roles in ischemia/reperfusion (I/R) cerebral injuries. Microglia activation has been shown to be closely related to TLR4/NF-κB signal pathways. Salvianolic acids for injection (SAFI) have been used in clinical practice to treat ischemic stroke with reported neuroprotective effects; however, the underlying mechanisms are still uncertain. Objective and Methods First, we studied the effect of SAFI on inflammatory responses in LPS-stimulated BV-2 microglia. Then, to discover whether the beneficial in vitro effects of SAFI lead to in vivo therapeutic effects, an MCAO (Middle cerebral artery occlusion) rat model was further employed to elucidate the probable mechanism of SAFI in treating ischemic stroke. Rats in the SAFI group were given SAFI (23 or 46 mg/kg) before I/R injury. Results The results showed that SAFI treatment significantly decreased neuroinflammation and the infarction volume compared with the vehicle group. Activation of microglia cells was reduced, and TLR4/NF-κB signals, which were markedly inhibited by SAFI treatment in ischemic hemisphere, were accompanied by reduced expression and release of cytokines IL-1β and IL-6. Conclusion This study provides evidence that SAFI effectively protects the brain after cerebral ischemia, which may be caused by attenuating inflammation in microglia.
AB - Background Inflammatory reactions induced by microglia in the brain play crucial roles in ischemia/reperfusion (I/R) cerebral injuries. Microglia activation has been shown to be closely related to TLR4/NF-κB signal pathways. Salvianolic acids for injection (SAFI) have been used in clinical practice to treat ischemic stroke with reported neuroprotective effects; however, the underlying mechanisms are still uncertain. Objective and Methods First, we studied the effect of SAFI on inflammatory responses in LPS-stimulated BV-2 microglia. Then, to discover whether the beneficial in vitro effects of SAFI lead to in vivo therapeutic effects, an MCAO (Middle cerebral artery occlusion) rat model was further employed to elucidate the probable mechanism of SAFI in treating ischemic stroke. Rats in the SAFI group were given SAFI (23 or 46 mg/kg) before I/R injury. Results The results showed that SAFI treatment significantly decreased neuroinflammation and the infarction volume compared with the vehicle group. Activation of microglia cells was reduced, and TLR4/NF-κB signals, which were markedly inhibited by SAFI treatment in ischemic hemisphere, were accompanied by reduced expression and release of cytokines IL-1β and IL-6. Conclusion This study provides evidence that SAFI effectively protects the brain after cerebral ischemia, which may be caused by attenuating inflammation in microglia.
KW - Inflammatory reaction
KW - Ischemia/Reperfusion cerebral injury
KW - Microglia
KW - Salvianolic Acids for Injection
KW - TLR4/NF-κB signals
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U2 - 10.1016/j.jep.2016.11.052
DO - 10.1016/j.jep.2016.11.052
M3 - Article
C2 - 28087473
AN - SCOPUS:85009513520
SN - 0378-8741
VL - 198
SP - 194
EP - 204
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
ER -