Scene-based Shack-Hartmann wavefront sensor for light-sheet microscopy

Keelan Lawrence; Yang Liu; Savannah Dale; Rebecca Ball; Ariel J. Vanleuven; Andrew Sornborger; James D. Lauderdale; Peter Kner

doi:10.1117/12.2288777

Scene-based Shack-Hartmann wavefront sensor for light-sheet microscopy

Keelan Lawrence, Yang Liu, Savannah Dale, Rebecca Ball, Ariel J. Vanleuven, Andrew Sornborger, James D. Lauderdale, Peter Kner

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

7 Scopus citations

Abstract

Light-sheet microscopy is an ideal imaging modality for long-term live imaging in model organisms. However, significant optical aberrations can be present when imaging into an organism that is hundreds of microns or greater in size. To measure and correct optical aberrations, an adaptive optics system must be incorporated into the microscope. Many biological samples lack point sources that can be used as guide stars with conventional Shack-Hartmann wavefront sensors. We have developed a scene-based Shack-Hartmann wavefront sensor for measuring the optical aberrations in a light-sheet microscopy system that does not require a point-source and can measure the aberrations for different parts of the image. The sensor has 280 lenslets inside the pupil, creates an image from each lenslet with a 500 micron field of view and a resolution of 8 microns, and has a resolution for the wavefront gradient of 75 milliradians per lenslet. We demonstrate the system on both fluorescent bead samples and zebrafish embryos.

Original language	English (US)
Title of host publication	Adaptive Optics and Wavefront Control for Biological Systems IV
Editors	Joel Kubby, Sylvain Gigan, Thomas G. Bifano
Publisher	SPIE
ISBN (Electronic)	9781510614895
DOIs	https://doi.org/10.1117/12.2288777
State	Published - 2018
Externally published	Yes
Event	Adaptive Optics and Wavefront Control for Biological Systems IV 2018 - San Francisco, United States Duration: Jan 27 2018 → Jan 29 2018

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10502
ISSN (Print)	1605-7422

Conference

Conference	Adaptive Optics and Wavefront Control for Biological Systems IV 2018
Country/Territory	United States
City	San Francisco
Period	1/27/18 → 1/29/18

Keywords

Adaptive Optics
Fluorescence Microscopy
Light Sheet Microscopy
Wavefront Sensing

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2288777

Cite this

Lawrence, K., Liu, Y., Dale, S., Ball, R., Vanleuven, A. J., Sornborger, A., Lauderdale, J. D., & Kner, P. (2018). Scene-based Shack-Hartmann wavefront sensor for light-sheet microscopy. In J. Kubby, S. Gigan, & T. G. Bifano (Eds.), Adaptive Optics and Wavefront Control for Biological Systems IV Article 105020B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10502). SPIE. https://doi.org/10.1117/12.2288777

Scene-based Shack-Hartmann wavefront sensor for light-sheet microscopy. / Lawrence, Keelan; Liu, Yang; Dale, Savannah et al.
Adaptive Optics and Wavefront Control for Biological Systems IV. ed. / Joel Kubby; Sylvain Gigan; Thomas G. Bifano. SPIE, 2018. 105020B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10502).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lawrence, K, Liu, Y, Dale, S, Ball, R, Vanleuven, AJ, Sornborger, A, Lauderdale, JD & Kner, P 2018, Scene-based Shack-Hartmann wavefront sensor for light-sheet microscopy. in J Kubby, S Gigan & TG Bifano (eds), Adaptive Optics and Wavefront Control for Biological Systems IV., 105020B, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10502, SPIE, Adaptive Optics and Wavefront Control for Biological Systems IV 2018, San Francisco, United States, 1/27/18. https://doi.org/10.1117/12.2288777

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Scene-based Shack-Hartmann wavefront sensor for light-sheet microscopy

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

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