Screening for the breast cancer gene (BRCA1) using a biochip system and molecular beacon probes immobilized on solid surfaces

Mustafa Culha, David L. Stokes, Guy D. Griffin, Tuan Vo-Dinh

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We describe the use of a biochip based on complementary metal oxide semiconductor (CMOS) technology for detection of specific genetic sequences using molecular beacons (MB) immobilized on solid surfaces as probes. The applicability of this miniature detection system for screening for the BRCA1 gene is evaluated using MB probes, designed especially for the BRCA1 gene. MB probes are immobilized on a zeta-probe membrane by biotin-streptavidin immobilization. Two immobilization strategies are investigated to obtain optimal assay sensitivity. The MB is immobilized by manual spotting on zeta-probe membrane surfaces with the use of a custom-made stamping system. The detection of the BRCA1 gene using an MB probe is successfully demonstrated and expands the use of the CMOS biochip for medical applications.

Original languageEnglish (US)
Pages (from-to)439-443
Number of pages5
JournalJournal of Biomedical Optics
Volume9
Issue number3
DOIs
StatePublished - May 2004
Externally publishedYes

Keywords

  • BRCA1
  • Biochip
  • Detection
  • Fluorescence
  • Molecular beacon

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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