Abstract
Berardinelli-Seip congenital lipodystrophy 2-deficient (Bscl2-/-) mice recapitulate human BSCL2 disease with lipodystrophy. Bscl2-encoded seipin is detected in adipocytes and epithelium of mammary gland. Postnatal mammary gland growth spurt and vaginal opening signify pubertal onset in female mice. Bscl2-/- females have longer and dilated mammary gland ducts at 5-week old and delayed vaginal opening. Prepubertal exposure to 500ppm genistein diet increases mammary gland area and accelerates vaginal opening in both control and Bscl2-/- females. However, genistein treatment increases ductal length in control but not Bscl2-/- females. Neither prepubertal genistein treatment nor Bscl2-deficiency affects phospho-estrogen receptor α or progesterone receptor expression patterns in 5-week old mammary gland. Interestingly, Bscl2-deficiency specifically reduces estrogen receptor β expression in mammary gland ductal epithelium. In summary, Bscl2-/- females have accelerated postnatal mammary ductal development but delayed vaginal opening; they display segregated responses in mammary gland development and vaginal opening to prepubertal genistein treatment.
Original language | English (US) |
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Pages (from-to) | 76-83 |
Number of pages | 8 |
Journal | Reproductive Toxicology |
Volume | 54 |
DOIs | |
State | Published - Jul 1 2015 |
Keywords
- Bscl2/Seipin
- Genistein
- Lipodystrophy
- Mammary gland
- Puberty
- Vaginal opening
ASJC Scopus subject areas
- Toxicology