Self-contamination of deep dentin by dentin fluid

Anut Itthagarun, Franklin Chi Meng Tay

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


Purpose: To compare, with the use of a resin replica technique, surface features of deep, acid-conditioned dentin from vital human molars that were anesthetized with an anesthetic: Group 1: without a vasoconstrictor (Mepivacaine 3%), and Group 2: containing a vasoconstrictor (Lidocaine 2% with 1:80,000 epinephrine). Materials and Methods: 10 Class I cavities with dentin caries were included in each group. Following complete caries removal, a total-etch technique was performed with 32% phosphoric acid (Uni-Etch) for 15 s. After rinsing, each cavity was air-dried for 1 s, then a slow setting vinyl polysiloxane (President) impression was taken. As a control, impressions were taken from three additional cavities in teeth that were anesthetized with Mepivacaine 3% but not acid etched. A TEM-grade epoxy resin was used to prepare replicas from the impressions. Polymerized replicas were coated with gold and examined with a scanning electron microscope. Results: In Group 1, fluid was visible leaving tubular orifices in all replicas. In addition, three were covered with a smooth, amorphous film that was different from the granular appearance of the smear layer in the control cavities. In Group 2, patent tubular orifices without fluid were observed in all specimens. Odontoblast process-like structures were seen from some dentin tubules.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalAmerican Journal of Dentistry
Issue number4
StatePublished - Aug 1 2000
Externally publishedYes

ASJC Scopus subject areas

  • Dentistry(all)


Dive into the research topics of 'Self-contamination of deep dentin by dentin fluid'. Together they form a unique fingerprint.

Cite this