Self-healing adhesive with antibacterial activity in water-aging for 12 months

Junling Wu; Chuanjian Zhou; Jianping Ruan; Michael D. Weir; Franklin Chi Meng Tay; Jirun Sun; Mary Anne S. Melo; Thomas W. Oates; Xiaofeng Chang; Hockin H.K. Xu

doi:10.1016/j.dental.2019.05.004

Self-healing adhesive with antibacterial activity in water-aging for 12 months

Junling Wu, Chuanjian Zhou, Jianping Ruan, Michael D. Weir, Franklin Chi Meng Tay, Jirun Sun, Mary Anne S. Melo, Thomas W. Oates, Xiaofeng Chang, Hockin H.K. Xu

Endodontics

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Objective: Secondary caries and micro-cracks are the main limiting factors for dentin bond durability. The objectives of this study were to develop a self-healing adhesive containing dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP), and investigate the effects of water-aging for 12 months on self-healing, dentin bonding, and antibacterial properties for the first time. Methods: Microcapsules were synthesized with poly (urea-formaldehyde) (PUF) shells containing triethylene glycol dimethacrylate (TEGDMA) and N,N-dihydroxyethyl-p-toluidine (DHEPT). The adhesive contained 7.5% microcapsules, 10% DMAHDM, and 20% NACP (all mass). Specimens were water-aged at 37 °C for 1 day to 12 months. Dentin bond strength was measured using extracted human teeth. A single-edge-V-notched-beam (SEVNB) method was used to measure fracture toughness K_IC and self-healing efficiency. A dental plaque microcosm biofilm model was used with human saliva as inoculum. Results: The microcapsules + DMAHDM + NACP group showed no decline in dentin bond strength after water-aging for 12 months, which was significantly higher than that of other groups without DMAHDM (p < 0.05). A self-healing efficiency of 67% recovery in K_IC was obtained even after 12 months of water immersion, indicating that the self-healing ability was not lost in water-aging (p > 0.1). The bacteria-killing ability of this adhesive did not decline from 1 day to 12 months (p > 0.1), with biofilm CFU reduction by 3–4 orders of magnitude after the resin was water-aged for 12 months, compared to control resin. Significance: This novel adhesive with triple merits of self-healing, antibacterial and remineralization functions showed an excellent long-term durability in water-aging for 12 months. This multifunctional adhesive has the potential for dental applications to heal cracks, inhibit bacteria, provide ions for remineralization, and increase the restoration longevity.

Original language	English (US)
Pages (from-to)	1104-1116
Number of pages	13
Journal	Dental Materials
Volume	35
Issue number	8
DOIs	https://doi.org/10.1016/j.dental.2019.05.004
State	Published - Aug 2019

Keywords

Antimicrobial
Dental adhesive
Microcapsules
Remineralization
Self-healing
Water-aging

ASJC Scopus subject areas

Materials Science(all)
Dentistry(all)
Mechanics of Materials

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10.1016/j.dental.2019.05.004

Cite this

@article{7112478cc5fc48599ece78e0b8d3f780,

title = "Self-healing adhesive with antibacterial activity in water-aging for 12 months",

abstract = "Objective: Secondary caries and micro-cracks are the main limiting factors for dentin bond durability. The objectives of this study were to develop a self-healing adhesive containing dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP), and investigate the effects of water-aging for 12 months on self-healing, dentin bonding, and antibacterial properties for the first time. Methods: Microcapsules were synthesized with poly (urea-formaldehyde) (PUF) shells containing triethylene glycol dimethacrylate (TEGDMA) and N,N-dihydroxyethyl-p-toluidine (DHEPT). The adhesive contained 7.5% microcapsules, 10% DMAHDM, and 20% NACP (all mass). Specimens were water-aged at 37 °C for 1 day to 12 months. Dentin bond strength was measured using extracted human teeth. A single-edge-V-notched-beam (SEVNB) method was used to measure fracture toughness KIC and self-healing efficiency. A dental plaque microcosm biofilm model was used with human saliva as inoculum. Results: The microcapsules + DMAHDM + NACP group showed no decline in dentin bond strength after water-aging for 12 months, which was significantly higher than that of other groups without DMAHDM (p < 0.05). A self-healing efficiency of 67% recovery in KIC was obtained even after 12 months of water immersion, indicating that the self-healing ability was not lost in water-aging (p > 0.1). The bacteria-killing ability of this adhesive did not decline from 1 day to 12 months (p > 0.1), with biofilm CFU reduction by 3–4 orders of magnitude after the resin was water-aged for 12 months, compared to control resin. Significance: This novel adhesive with triple merits of self-healing, antibacterial and remineralization functions showed an excellent long-term durability in water-aging for 12 months. This multifunctional adhesive has the potential for dental applications to heal cracks, inhibit bacteria, provide ions for remineralization, and increase the restoration longevity.",

keywords = "Antimicrobial, Dental adhesive, Microcapsules, Remineralization, Self-healing, Water-aging",

author = "Junling Wu and Chuanjian Zhou and Jianping Ruan and Weir, {Michael D.} and Tay, {Franklin Chi Meng} and Jirun Sun and Melo, {Mary Anne S.} and Oates, {Thomas W.} and Xiaofeng Chang and Xu, {Hockin H.K.}",

note = "Funding Information: This study was supported by National Natural Science Foundation (NSF) of China 81671032 (JLW), a seed grant from the University of Maryland Baltimore (HX) , and a bridge grant from the University of Maryland School of Dentistry (HX) .",

year = "2019",

month = aug,

doi = "10.1016/j.dental.2019.05.004",

language = "English (US)",

volume = "35",

pages = "1104--1116",

journal = "Dental Materials",

issn = "0109-5641",

publisher = "Elsevier Science",

number = "8",

}

TY - JOUR

T1 - Self-healing adhesive with antibacterial activity in water-aging for 12 months

AU - Wu, Junling

AU - Zhou, Chuanjian

AU - Ruan, Jianping

AU - Weir, Michael D.

AU - Tay, Franklin Chi Meng

AU - Sun, Jirun

AU - Melo, Mary Anne S.

AU - Oates, Thomas W.

AU - Chang, Xiaofeng

AU - Xu, Hockin H.K.

N1 - Funding Information: This study was supported by National Natural Science Foundation (NSF) of China 81671032 (JLW), a seed grant from the University of Maryland Baltimore (HX) , and a bridge grant from the University of Maryland School of Dentistry (HX) .

PY - 2019/8

Y1 - 2019/8

N2 - Objective: Secondary caries and micro-cracks are the main limiting factors for dentin bond durability. The objectives of this study were to develop a self-healing adhesive containing dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP), and investigate the effects of water-aging for 12 months on self-healing, dentin bonding, and antibacterial properties for the first time. Methods: Microcapsules were synthesized with poly (urea-formaldehyde) (PUF) shells containing triethylene glycol dimethacrylate (TEGDMA) and N,N-dihydroxyethyl-p-toluidine (DHEPT). The adhesive contained 7.5% microcapsules, 10% DMAHDM, and 20% NACP (all mass). Specimens were water-aged at 37 °C for 1 day to 12 months. Dentin bond strength was measured using extracted human teeth. A single-edge-V-notched-beam (SEVNB) method was used to measure fracture toughness KIC and self-healing efficiency. A dental plaque microcosm biofilm model was used with human saliva as inoculum. Results: The microcapsules + DMAHDM + NACP group showed no decline in dentin bond strength after water-aging for 12 months, which was significantly higher than that of other groups without DMAHDM (p < 0.05). A self-healing efficiency of 67% recovery in KIC was obtained even after 12 months of water immersion, indicating that the self-healing ability was not lost in water-aging (p > 0.1). The bacteria-killing ability of this adhesive did not decline from 1 day to 12 months (p > 0.1), with biofilm CFU reduction by 3–4 orders of magnitude after the resin was water-aged for 12 months, compared to control resin. Significance: This novel adhesive with triple merits of self-healing, antibacterial and remineralization functions showed an excellent long-term durability in water-aging for 12 months. This multifunctional adhesive has the potential for dental applications to heal cracks, inhibit bacteria, provide ions for remineralization, and increase the restoration longevity.

AB - Objective: Secondary caries and micro-cracks are the main limiting factors for dentin bond durability. The objectives of this study were to develop a self-healing adhesive containing dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP), and investigate the effects of water-aging for 12 months on self-healing, dentin bonding, and antibacterial properties for the first time. Methods: Microcapsules were synthesized with poly (urea-formaldehyde) (PUF) shells containing triethylene glycol dimethacrylate (TEGDMA) and N,N-dihydroxyethyl-p-toluidine (DHEPT). The adhesive contained 7.5% microcapsules, 10% DMAHDM, and 20% NACP (all mass). Specimens were water-aged at 37 °C for 1 day to 12 months. Dentin bond strength was measured using extracted human teeth. A single-edge-V-notched-beam (SEVNB) method was used to measure fracture toughness KIC and self-healing efficiency. A dental plaque microcosm biofilm model was used with human saliva as inoculum. Results: The microcapsules + DMAHDM + NACP group showed no decline in dentin bond strength after water-aging for 12 months, which was significantly higher than that of other groups without DMAHDM (p < 0.05). A self-healing efficiency of 67% recovery in KIC was obtained even after 12 months of water immersion, indicating that the self-healing ability was not lost in water-aging (p > 0.1). The bacteria-killing ability of this adhesive did not decline from 1 day to 12 months (p > 0.1), with biofilm CFU reduction by 3–4 orders of magnitude after the resin was water-aged for 12 months, compared to control resin. Significance: This novel adhesive with triple merits of self-healing, antibacterial and remineralization functions showed an excellent long-term durability in water-aging for 12 months. This multifunctional adhesive has the potential for dental applications to heal cracks, inhibit bacteria, provide ions for remineralization, and increase the restoration longevity.

KW - Antimicrobial

KW - Dental adhesive

KW - Microcapsules

KW - Remineralization

KW - Self-healing

KW - Water-aging

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ER -

Self-healing adhesive with antibacterial activity in water-aging for 12 months

Abstract

Keywords

ASJC Scopus subject areas

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