Sensitive liquid chromatography/tandem mass spectrometry method for the determination of two novel highly lipophilic anticancer drug candidates in rat plasma and tissues

Shirin Hooshfar; Michael R. Linzey; Daqing Wu; Lajos Gera; Kenza Mamouni; Xin Li; Yanhua Chen; Yang Yang; Oluwasegun Olorunyolemi; Michael G. Bartlett

doi:10.1002/bmc.4064

Sensitive liquid chromatography/tandem mass spectrometry method for the determination of two novel highly lipophilic anticancer drug candidates in rat plasma and tissues

Shirin Hooshfar, Michael R. Linzey, Daqing Wu, Lajos Gera, Kenza Mamouni, Xin Li, Yanhua Chen, Yang Yang, Oluwasegun Olorunyolemi, Michael G. Bartlett

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A simple and sensitive liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for determination of two highly lipophilic anticancer drug candidates, LG1980 and GH501, in rat plasma and tissues (liver, kidney and femur bones). LG1980 and GH501 were extracted from rat plasma and tissue homogenates using liquid–liquid extraction. The method provided a linear range of 1.0–200.0 ng/mL for GH501 in plasma and LG1980 in plasma and liver. For both analytes in other tissue homogenates the linear range was 2.0–400.0 ng/mL. The method was validated with precision within 15% relative standard deviation, accuracy within 15% relative error and a consistent recovery. This method has been successfully applied in two preclinical studies for LG1980 and GH501 to determine their concentrations in rat plasma, liver, kidney and bone over 24 h after intravenous injection of compounds.

Original language	English (US)
Article number	e4064
Journal	Biomedical Chromatography
Volume	32
Issue number	2
DOIs	https://doi.org/10.1002/bmc.4064
State	Published - Feb 2018

Keywords

LC–MS/MS
bioanalysis
highly lipophilic drug
plasma
tissue

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

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Hooshfar, S., Linzey, M. R., Wu, D., Gera, L., Mamouni, K., Li, X., Chen, Y., Yang, Y., Olorunyolemi, O., & Bartlett, M. G. (2018). Sensitive liquid chromatography/tandem mass spectrometry method for the determination of two novel highly lipophilic anticancer drug candidates in rat plasma and tissues. Biomedical Chromatography, 32(2), Article e4064. https://doi.org/10.1002/bmc.4064

Hooshfar, S, Linzey, MR, Wu, D, Gera, L, Mamouni, K, Li, X, Chen, Y, Yang, Y, Olorunyolemi, O & Bartlett, MG 2018, 'Sensitive liquid chromatography/tandem mass spectrometry method for the determination of two novel highly lipophilic anticancer drug candidates in rat plasma and tissues', Biomedical Chromatography, vol. 32, no. 2, e4064. https://doi.org/10.1002/bmc.4064

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abstract = "A simple and sensitive liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for determination of two highly lipophilic anticancer drug candidates, LG1980 and GH501, in rat plasma and tissues (liver, kidney and femur bones). LG1980 and GH501 were extracted from rat plasma and tissue homogenates using liquid–liquid extraction. The method provided a linear range of 1.0–200.0 ng/mL for GH501 in plasma and LG1980 in plasma and liver. For both analytes in other tissue homogenates the linear range was 2.0–400.0 ng/mL. The method was validated with precision within 15% relative standard deviation, accuracy within 15% relative error and a consistent recovery. This method has been successfully applied in two preclinical studies for LG1980 and GH501 to determine their concentrations in rat plasma, liver, kidney and bone over 24 h after intravenous injection of compounds.",

keywords = "LC–MS/MS, bioanalysis, highly lipophilic drug, plasma, tissue",

author = "Shirin Hooshfar and Linzey, {Michael R.} and Daqing Wu and Lajos Gera and Kenza Mamouni and Xin Li and Yanhua Chen and Yang Yang and Oluwasegun Olorunyolemi and Bartlett, {Michael G.}",

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doi = "10.1002/bmc.4064",

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AU - Hooshfar, Shirin

AU - Linzey, Michael R.

AU - Wu, Daqing

AU - Gera, Lajos

AU - Mamouni, Kenza

AU - Li, Xin

AU - Chen, Yanhua

AU - Yang, Yang

AU - Olorunyolemi, Oluwasegun

AU - Bartlett, Michael G.

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N2 - A simple and sensitive liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for determination of two highly lipophilic anticancer drug candidates, LG1980 and GH501, in rat plasma and tissues (liver, kidney and femur bones). LG1980 and GH501 were extracted from rat plasma and tissue homogenates using liquid–liquid extraction. The method provided a linear range of 1.0–200.0 ng/mL for GH501 in plasma and LG1980 in plasma and liver. For both analytes in other tissue homogenates the linear range was 2.0–400.0 ng/mL. The method was validated with precision within 15% relative standard deviation, accuracy within 15% relative error and a consistent recovery. This method has been successfully applied in two preclinical studies for LG1980 and GH501 to determine their concentrations in rat plasma, liver, kidney and bone over 24 h after intravenous injection of compounds.

AB - A simple and sensitive liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for determination of two highly lipophilic anticancer drug candidates, LG1980 and GH501, in rat plasma and tissues (liver, kidney and femur bones). LG1980 and GH501 were extracted from rat plasma and tissue homogenates using liquid–liquid extraction. The method provided a linear range of 1.0–200.0 ng/mL for GH501 in plasma and LG1980 in plasma and liver. For both analytes in other tissue homogenates the linear range was 2.0–400.0 ng/mL. The method was validated with precision within 15% relative standard deviation, accuracy within 15% relative error and a consistent recovery. This method has been successfully applied in two preclinical studies for LG1980 and GH501 to determine their concentrations in rat plasma, liver, kidney and bone over 24 h after intravenous injection of compounds.

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KW - bioanalysis

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KW - plasma

KW - tissue

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Sensitive liquid chromatography/tandem mass spectrometry method for the determination of two novel highly lipophilic anticancer drug candidates in rat plasma and tissues

Abstract

Keywords

ASJC Scopus subject areas

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