TY - JOUR
T1 - Separation of normal and abnormal hemoglobin chains by reversed-phase high-performance liquid chromatography
AU - Kutlar, F.
AU - Kutlar, A.
AU - Huisman, T. H.J.
PY - 1986
Y1 - 1986
N2 - A review is presented of the elution patterns on reversed-phase columns of the normal and abnormal globin chains of different hemoglobin types, including 16 β-chain variants, 7 α-chain variants, 9 γ-chain variants, and 4-variants with fusion or hybrid chains. Separations appear to be based primarily on differences in hydrophobicity. The method is ideally suited for the detection of abnormal globin chains, their quantitation and their isolation. Semi-quantitative data based on the calculation of the δ non-α ratios allow the detection of β-thalassemic conditions in situations where the quantitation of hemoglobin A2 by other procedures is impossible or complicated.
AB - A review is presented of the elution patterns on reversed-phase columns of the normal and abnormal globin chains of different hemoglobin types, including 16 β-chain variants, 7 α-chain variants, 9 γ-chain variants, and 4-variants with fusion or hybrid chains. Separations appear to be based primarily on differences in hydrophobicity. The method is ideally suited for the detection of abnormal globin chains, their quantitation and their isolation. Semi-quantitative data based on the calculation of the δ non-α ratios allow the detection of β-thalassemic conditions in situations where the quantitation of hemoglobin A2 by other procedures is impossible or complicated.
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U2 - 10.1016/S0021-9673(01)95816-9
DO - 10.1016/S0021-9673(01)95816-9
M3 - Article
C2 - 3711181
AN - SCOPUS:0022460617
SN - 0021-9673
VL - 357
SP - 147
EP - 153
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - C
ER -