TY - JOUR
T1 - Sequence specific interaction of the chromosomal proteins with DNA
AU - Tuan, Dorothy
AU - Chetsanga, Christopher
AU - Doty, Paul M.
N1 - Funding Information:
Supported by National Institutes of
PY - 1977/10
Y1 - 1977/10
N2 - Calf thymus chromatin was partially deproteinized by 0.6-1 M NaCl extraction. From the shape of the temperature derivative plot of its melting curve, the DNA in each chromatin was resolved into regions of exposed DNA, and DNA still complexed with proteins. The partially deproteinized chromatin was capable of directing in vitroRNA synthesis in an amount proportional to the fraction of exposed DNA. The in vitro RNA transcript was hybridized to denatured calf DNA under hybridization conditions where only RNA's transcribed from repetitive DNA sequences were able to from hybrids. From the results, it is deduced that the in vitroRNA transcripts of 0.6 M NaCl-extracted chromatin after the removal of HI histones plus one-quarter of the non histones may be transcribed from repetitive DNA sequences and those of 1 M NaCl-extracted chromatin contain more transcripts from the unique DNA sequences.
AB - Calf thymus chromatin was partially deproteinized by 0.6-1 M NaCl extraction. From the shape of the temperature derivative plot of its melting curve, the DNA in each chromatin was resolved into regions of exposed DNA, and DNA still complexed with proteins. The partially deproteinized chromatin was capable of directing in vitroRNA synthesis in an amount proportional to the fraction of exposed DNA. The in vitro RNA transcript was hybridized to denatured calf DNA under hybridization conditions where only RNA's transcribed from repetitive DNA sequences were able to from hybrids. From the results, it is deduced that the in vitroRNA transcripts of 0.6 M NaCl-extracted chromatin after the removal of HI histones plus one-quarter of the non histones may be transcribed from repetitive DNA sequences and those of 1 M NaCl-extracted chromatin contain more transcripts from the unique DNA sequences.
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U2 - 10.1093/nar/4.10.3415
DO - 10.1093/nar/4.10.3415
M3 - Article
C2 - 337237
AN - SCOPUS:0017671025
SN - 0305-1048
VL - 4
SP - 3415
EP - 3439
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 10
ER -