TY - JOUR
T1 - Shear-dependent inhibition of granulocyte adhesion to cultured endothelium by dextran sulfate
AU - Ley, K.
AU - Lundgren, E.
AU - Berger, E.
AU - Arfors, K. E.
PY - 1989
Y1 - 1989
N2 - Adhesion of polymorphonuclear granulocytes (PMNs) in microvessels occurs in the presence of shear forces exerted by the blood flow. To model this in vitro, phorbol myristate acetate (PMA)-activated PMN were exposed to shear stress on cultured human umbilical vein endothelial cells (HUVECs) and on plastic dishes coated with bovine serum albumin (BSA). PMN adhesion to HUVECs averaged 36% of the total PMNs added and was reduced to 21% by shear stress of ~ 1.5 dynes · cm-2. On BSA, adhesion was reduced from 59% to 35%. Dextran sulfate (molecular weight 500,000) inhibited PMN adhesion in a dose-dependent manner when shear stress was applied. At a concentration of 1 mg · ml-1, inhibition was 72% on HUVECs and 76% on BSA. Half-maximal inhibition was reached at ~ 1 μg · mL-1 dextran sulfate, corresponding to 2 nmol/L. Without shear stress, dextran sulfate had no effect on HUVECs and only a moderate effect on BSA. The murine monoclonal antibody (MoAb) 60.3, recognizing an epitope on the leukocyte adhesion glycoprotein CD18, inhibited PMN adhesion equally well with and without shear. A low dose of MoAb 60.3 enhanced the effect of dextran sulfate without shear stress. Flow cytometry (FACS) did not show inhibition of MoAb 60.3 binding to PMNs by dextran sulfate. These reuslts indicate that a dextran sulfate-inhibitable adhesion process is important for PMN adhesion in the presence of shear stress.
AB - Adhesion of polymorphonuclear granulocytes (PMNs) in microvessels occurs in the presence of shear forces exerted by the blood flow. To model this in vitro, phorbol myristate acetate (PMA)-activated PMN were exposed to shear stress on cultured human umbilical vein endothelial cells (HUVECs) and on plastic dishes coated with bovine serum albumin (BSA). PMN adhesion to HUVECs averaged 36% of the total PMNs added and was reduced to 21% by shear stress of ~ 1.5 dynes · cm-2. On BSA, adhesion was reduced from 59% to 35%. Dextran sulfate (molecular weight 500,000) inhibited PMN adhesion in a dose-dependent manner when shear stress was applied. At a concentration of 1 mg · ml-1, inhibition was 72% on HUVECs and 76% on BSA. Half-maximal inhibition was reached at ~ 1 μg · mL-1 dextran sulfate, corresponding to 2 nmol/L. Without shear stress, dextran sulfate had no effect on HUVECs and only a moderate effect on BSA. The murine monoclonal antibody (MoAb) 60.3, recognizing an epitope on the leukocyte adhesion glycoprotein CD18, inhibited PMN adhesion equally well with and without shear. A low dose of MoAb 60.3 enhanced the effect of dextran sulfate without shear stress. Flow cytometry (FACS) did not show inhibition of MoAb 60.3 binding to PMNs by dextran sulfate. These reuslts indicate that a dextran sulfate-inhibitable adhesion process is important for PMN adhesion in the presence of shear stress.
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U2 - 10.1182/blood.v73.5.1324.bloodjournal7351324
DO - 10.1182/blood.v73.5.1324.bloodjournal7351324
M3 - Article
C2 - 2467707
AN - SCOPUS:0024556763
SN - 0006-4971
VL - 73
SP - 1324
EP - 1330
JO - Blood
JF - Blood
IS - 5
ER -